Purified anti-human CD10 (Maxpar® Ready) Antibody

Pricing & Availability
Clone
HI10a (See other available formats)
Workshop
V CD10.7
Other Names
Common acute lymphoblastic leukemia antigen (CALLA), Enkephalinase, Neutral endopeptidase, Neprilysin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Hi10a_Purified_CD10_CyTOF_112213
Human PBMCs (top) and human NALM-6 pre-B cells (bottom) stained with 156Gdanti-CD10 (HI10a). Data provided by DVS Sciences.
  • Hi10a_Purified_CD10_CyTOF_112213
    Human PBMCs (top) and human NALM-6 pre-B cells (bottom) stained with 156Gdanti-CD10 (HI10a). Data provided by DVS Sciences.
Cat # Size Price Quantity Avail. Save
312223 100 µg $120
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Description

CD10 is a 100 kD neutral endopeptidase and a member of the metalloprotease family. It is a type II transmembrane protein also known as common acute lymphoblastic leukemia antigen (CALLA), enkephalinase, and neprilysin. CD10 is expressed on B cell precursors, T cell precursors, and neutrophils. CD10 is involved in B cell development and has been shown to bind opioid enkephalins, bradykinin, angiotensins I and II, and other biologically active peptides.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Capuchin monkey, Chimpanzee, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Validated

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated so that the buffer exchange step is not required (steps 7, 8, 9, and 10 in the Maxpar® antibody labeling protocol). Just add 100 µl of this antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter, as described in step 11, and continue with the protocol.

Additional Product Notes

Maxpar® is a registered trademark of Fluidigm Inc.

RRID
AB_2562828 (BioLegend Cat. No. 312223)

Antigen Details

Structure
Type II transmembrane metalloprotease family, 100 kD
Distribution

B cell precursors, T cell precursors, neutrophils

Function
Zinc-binding metalloproteinase, B cell development
Ligand/Receptor
Biologically active peptides including opioid enkephalins, bradykinin, angiotensions I & II
Cell Type
B cells, Neutrophils
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Shipp M, et al. 1993. Blood 82:1052.
2. Lu B, et al. 1995. J. Exp. Med. 181:2271.

Gene ID
4311 View all products for this Gene ID
UniProt
View information about CD10 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 3    Revision Date: 03/05/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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