- Clone
- A21052D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BL-CAM, Siglec-2, Lyb8
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A21052D_PURE_CD22_Antibody_033022 A21052D_PURE_CD22_Antibody_033022](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A21052D_PURE_CD22_Antibody_033022.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD22 (clone A21052D) and purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG (clone Poly4053) (red) and Alexa Fluor® 647 goat anti-rat IgG (clone Poly4054) (green) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
382701 | 25 µg | $55 | ||||
382702 | 100 µg | $121 |
CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM and is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2927812 (BioLegend Cat. No. 382701)
AB_2927812 (BioLegend Cat. No. 382702)
Antigen Details
- Structure
- Ig superfamily, a type I glycosylated integral transmembrane protein, 120-130 kD
- Distribution
-
B cells
- Function
- Adhesion, B-monocyte and B-T interactions
- Ligand/Receptor
- CD45RO, CD75
- Biology Area
- Cell Adhesion
- Molecular Family
- Adhesion Molecules
- Antigen References
-
- Clark E. 1993. J Immunol. 150:4715-8.
- Shan D, et al. 1995. J Immunol. 154:4466-75.
- Gene ID
- 933 View all products for this Gene ID
- UniProt
- View information about CD22 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD22 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD22 | A21052D | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD22
Human paraffin-embedded tonsil tissue slices were prepared w...
Follow Us