- Clone
- QA17A51 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD67, CGM6, NCA-95, CEACAM8
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA17A51_PURE_CD66b_Antibody_070219 QA17A51_PURE_CD66b_Antibody_070219](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA17A51_PURE_CD66b_Antibody_073119_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood granulocytes were stained with CD66b (clone QA17A51) Purified (filled histogram) or Mouse IgG1, κ Purified isotype control (open histogram) followed by anti-mouse IgG1 PE -
Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with 1X Citric buffer pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66b (clone QA17A51) antibody overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG (clone poly4053) antibody (red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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396902 | 100 µg | $141 |
CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2814367 (BioLegend Cat. No. 396902)
Antigen Details
- Distribution
-
Granulocytes/neutrophils
- Function
- Cell adhesion, neutrophil activation
- Ligand/Receptor
- CD66b
- Cell Type
- Granulocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Kuijpers T, et al. 1993. J. Immunol. 151:4934
- Kuroki M, et al. 1992. J. Leuk. Biol. 52:551
- Gene ID
- 1088 View all products for this Gene ID
- UniProt
- View information about CD66b on UniProt.org
Other Formats
View All CD66b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human CD66b Recombinant Antibody | QA17A51 | FC |
Purified anti-human CD66b Recombinant Antibody | QA17A51 | FC,IHC-P |
APC/Fire™ 750 anti-human CD66b Recombinant Antibody | QA17A51 | FC |
PE/Dazzle™ 594 anti-human CD66b Recombinant Antibody | QA17A51 | FC |
PerCP/Cyanine5.5 anti-human CD66b Recombinant Antibody | QA17A51 | FC |
PE/Cyanine7 anti-human CD66b Recombinant Antibody | QA17A51 | FC |
APC anti-human CD66b Recombinant Antibody | QA17A51 | FC |
FITC anti-human CD66b Recombinant Antibody | QA17A51 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD66b Recombinant Antibody
Human peripheral blood was stained with CD15 APC and CD66b (... -
Purified anti-human CD66b Recombinant Antibody
Human peripheral blood granulocytes were stained with CD66b ... Human paraffin-embedded spleen tissue slices were prepared w... -
APC/Fire™ 750 anti-human CD66b Recombinant Antibody
Human peripheral blood was stained with True-Stain Monocyte ... -
PE/Dazzle™ 594 anti-human CD66b Recombinant Antibody
Human peripheral blood was stained with True-Stain Monocyte ... -
PerCP/Cyanine5.5 anti-human CD66b Recombinant Antibody
Human peripheral blood was stained with True-Stain Monocyte ... -
PE/Cyanine7 anti-human CD66b Recombinant Antibody
Human peripheral blood was stained with True-Stain Monocyte ... -
APC anti-human CD66b Recombinant Antibody
Human peripheral blood was stained with CD15 FITC and CD66b ... -
FITC anti-human CD66b Recombinant Antibody
Human peripheral blood granulocytes were stained with anti-h...
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