- Clone
- W18340A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ig delta chain C region
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_W18340A_PURE_IgD_Antibody_081220 A_W18340A_PURE_IgD_Antibody_081220](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W18340A_PURE_IgD_Antibody_081220.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, tissue was incubated with goat anti-rat IgG (Poly4054) Alexa Fluor® 594 antibody (red) and anti-human CD19 (clone A17136C) Alexa Fluor® 647 antibody (green) at room temperature for two hours. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with software. -
SeqIF™ (sequential immunofluorescence) staining on COMET™ of Purified anti-IgD (clone W18340A, yellow) on formalin-fixed paraffin-embedded human tonsil tissue at 5 µg/mL. Alexa Fluor™ Plus 555 Goat anti-Rat IgG antibody (Lunaphore, Cat. No. DR555RT) was used as a secondary antibody. Nuclei were counterstained with DAPI (blue). Tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
324502 | 100 µg | $54 |
IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full length human IgD recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
SB - Community verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
-
For the use of this antibody in spatial biology applications, we have partnered with Lunaphore Technologies for demonstration of our antibodies on the COMET™. The COMET™ platform is an automated, end-to-end spatial biology solution developed for rapid and flexible multiplex tissue profiling. More information on the COMET™ and a complete list of our antibodies that have been demonstrated on the COMET™ can be found here.
- RRID
-
AB_2888899 (BioLegend Cat. No. 324502)
Antigen Details
- Structure
- Exists in a transmembranal and a soluble form
- Distribution
-
Naïve B cells
- Function
- Antigen binding, B cell activation
- Interaction
- The CD79a/CD79b heterodimer
- Molecular Family
- Immune Checkpoint Receptors, Innate Immune Signaling
- Antigen References
-
- Geisberger R, et al. 2006. Immunology 118:429-37.
- Weller S, et al. 2005. Eur J Immunol. 35:2789-92.
- Brandtzaeg P & Johansen FE. 2005. Immunol Rev. 206:32-63.
- Gene ID
- 3495 View all products for this Gene ID
- UniProt
- View information about IgD on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
-
It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
-
Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
-
Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
-
Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
-
In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
View All IgD Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human IgD | W18340A | IHC-P,SB |
Alexa Fluor® 647 anti-human IgD | W18340A | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human IgD
Human paraffin-embedded tonsil tissue slices were prepared w... SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
Alexa Fluor® 647 anti-human IgD
IHC staining of anti-human IgD (clone W18340A) Alexa Fluor® ...
Follow Us