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Purified anti-human IL-32α Antibody

Pricing & Availability
Clone
KU32-07 (See other available formats)
Regulatory Status
RUO
Other Names
IL-32 alpha, Interleukin 32 alpha, Natural Killer Transcript 4 (NK4), TNF-α-inducible factor (TAIFa)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
IL-32alpha_Human_ELISA_121313
  • IL-32alpha_Human_ELISA_121313
Cat # Size Price Quantity Check Availability Save
513301 50 µg $136
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513302 500 µg $557
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Description

Interleukin 32 (IL-32), previously known as a transcript (NK4), is produced by mitogen-activated lymphocytes, by IFNγ-activated epithelial cells or by IL-12 and IL-18-activated NK cells. Its expression is increased following activation of T-cells by mitogens or the activation of NK cells by IL-2. IL-32 activates NF-κB and p38 MAPK cytokine signal pathways. It has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues. IL-32 exists in at least four differentially spliced isoforms (α, β, γ and δ) with predicted molecular weight: ~26 kD. IL-32α is the shortest and most abundant of four potential splice variants of the pro-inflammatory cytokine IL-32. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32α was more likely to be cell-associated as compared to IL-32β, suggesting an intracellular function.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-32α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-32 recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture1: The purified KU32-07 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated KU32-52 antibody (Cat. No. 513503) as the detecting antibody.

Note: For testing human IL-32a in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 433504 & 433505) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Kim KH, et al. 2008. J. Immunol. Methods 333:38. (ELISA Capture)
  2. Kulkarni O, et al. 2008. J. Pharmacol. Exp. Ther. 328:371. PubMed
  3. Greene CM, et al. 2010. AM J. Respir Crit Care Med. 181:31. PubMed
  4. Sakitani K, et al. 2012. Infect. Immun. 80:3795. (ELISA Capture) PubMed
  5. Li Y, et al. 2013. J. Biol. Chem. 288:20927. PubMed
RRID
AB_2561289 (BioLegend Cat. No. 513301)
AB_2561290 (BioLegend Cat. No. 513302)

Antigen Details

Structure
IL-32 contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence.
Distribution

Expression increased after activation of T-cells by mitogens or activation of NK cells by IL-2.

Function
IL-32 is a proinflammatory cytokine associated with viral and bacterial infections. IL-32 is involved in autoimmune diseases like rheumatoid arthritis and Crohn disease. IL-32 induces the production of TNF-a from macrophage cells.
Interaction
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia.
Ligand/Receptor
Proteinase 3 (PR3).
Cell Type
Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Kim KH, et al. 2008. J. Immunol. Methods 333:38.
2. Conti P, et al. 2007. Autoimmun. Rev. 6:131.
3. Chen Q, et al. 2006. Vitam Horm. 74:207.
4. Kim SH, et al. 2005. Immunity 22:131.
5. Cagnard N, et al. 2005. Eur. Cytokine Netw. 16:289.
6. Banda NK, et al. 2003. J. Immunol. 170:2100.

Gene ID
9235 View all products for this Gene ID
UniProt
View information about IL-32alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/22/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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  • Purified anti-human IL-32α

    IL-32alpha_Human_ELISA_121313
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