Purified anti-human TRA-1-81 Antibody

Pricing & Availability
Clone
TRA-1-81 (See other available formats)
Other Names
Podocalyxin
Isotype
Mouse IgM, κ
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Product Citations
publications
Tra-1-81_Pure_061208.jpg
Human teratocarcinoma cell line, NCCIT, stained with purified TRA-1-81, followed by anti-mouse IgM PE
  • Tra-1-81_Pure_061208.jpg
    Human teratocarcinoma cell line, NCCIT, stained with purified TRA-1-81, followed by anti-mouse IgM PE
  • TRA-1-81_Purified_WB_052314.jpg
    Western blot analysis of NTERA-2 (lane 1), NF-1 (lane 2) and Hela (lane 3) cells using anti-TRA-1-81 antibody (TRA-1-81). GAPDH (poly6314) was used a loading control.
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330702 100 µg $230
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Description

The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is a neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonic carcinoma cell line 2102Ep cl.2A6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB - Validated
IP, IF, IHC, ELISA - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence cell staining1, immunoprecipitation1, Western blotting1,2, ELISA1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Andrews PW, et al. 1984. Hybridoma. 3:347.
  2. Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
Product Citations
  1. Kamata M, et al. 2010. PLoS One. 5:e11834. PubMed
  2. Devine MJ, et al. 2011. Nat Commun. 1.263888889. PubMed
  3. Chen X, et al. 2017. Nat Commun. 10.1038/s41467-017-00687-1. PubMed
  4. Hey CAB et al. 2018. Stem cell research. 31:235-239 . PubMed
  5. McLenachan S et al. 2019. Stem Cell Res. 1723:17:00 . PubMed
  6. Claassen JN et al. 2019. Stem Cell Res. 1723:12:00 . PubMed
  7. Frederiksen HR et al. 2019. Stem cell research. 1725:03:00 . PubMed
  8. Frederiksen HR et al. 2019. Stem cell research. 1723:28:00 . PubMed
  9. Schmid B et al. 2019. Stem Cell Res. 1723:09:00 . PubMed
  10. Hey CAB et al. 2018. Stem Cell Res. 33:46-50 . PubMed
RRID
AB_1089240 (BioLegend Cat. No. 330702)

Antigen Details

Structure
Mucin-like protein, CD34 family member, 200-240 kD
Distribution
embryonic stem cells (ESC), embryonic germ cells (EG), embryonal carcinoma cells
Function
Anti-adhesion
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells
Antigen References

1. Andrews PW, et al. 1984. Hybridoma. 3:347.
2. Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
3. Takeda T, et al. 2000. Mol. Biol. Cell. 11:3219.

Gene ID
5420 View all products for this Gene ID
UniProt
View information about TRA-1-81 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 11/05/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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