- 2C9.G2 (HMβ3-1) (See other available formats)
- Other Names
- β3 integrin, Integrin β3 chain, gpIIIa
- Armenian Hamster IgG
- Ave. Rating
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- Product Citations
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CD61 is a 110 kD integrin β chain also known as β3 integrin or gpIIIa. It associates with the integrin αv chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin αIIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of αv/β3 integrin or vitronectin receptor to CD31 (PECAM-1).Product Details
- Mouse, Rat
- Antibody Type
- Host Species
- Armenian Hamster
- Vitronectin receptor protein from the mouse T-cell hybridoma 2B4.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of ligand binding1-4, activation of αvβ3 integrin signaling5, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 104310).
(PubMed link indicates BioLegend citation)
- Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block)
- Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
- Ashkar S, et al. 2000. Science 287:860. (Block)
- Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
- Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ)
- Carlson TR, et al. 2008.135:2193. PubMed
- Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed
- Product Citations
AB_313079 (BioLegend Cat. No. 104302)
- Integrin family, 110 kD
Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells
- Attachment to matrix
- Fibrinogen, von Willebrand factor, fibronectin
- Cell Type
- B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Phillips DR, et al. 1991. Cell 65:359.
3. Felding-Habermann B, et al. 1993. Curr. Opinion Cell Biol. 5:864.
- Gene ID
- 16416 View all products for this Gene ID 29302 View all products for this Gene ID
- View information about CD61 on UniProt.org
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