Purified anti-Nur77 Antibody

Pricing & Availability
Clone
1E10A15 (See other available formats)
Other Names
Nuclear receptor subfamily 4, group A, member 1 (NR4A1), GFRP, HMR, Testicular receptor 3 (TR3)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1E10A15_Purified_Nur77_Antibody_1_113018
Whole cell extracts (15 µg protein) from serum starved HeLa cells untreated (-) or treated (+) with 200 nM PMA for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:1000 dilution) of Purified anti-Nur77 Antibody, clone 1E10A15, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • 1E10A15_Purified_Nur77_Antibody_1_113018
    Whole cell extracts (15 µg protein) from serum starved HeLa cells untreated (-) or treated (+) with 200 nM PMA for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:1000 dilution) of Purified anti-Nur77 Antibody, clone 1E10A15, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
653501 25 µg $85
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653502 100 µg $225
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Description

Nur77, a transcription factor belonging to a superfamily of orphan nuclear receptors, has been shown to activate a subset of genes containing NGFI-B response element (NBRE). Nur77 expression is upregulated in response to stimuli of numerous growth factors, mitogens, and inflammatory substances, indicating that Nur77 has regulatory roles in cell growth and inflammation. Nur77 has also been shown to participate in programmed cell death through mitochondrial targeting and interaction with Bcl-2. In the endocrine system, Nur77 has been reported to participate in regulating production of steroidogenic enzymes. Nur77 and NOR-1 double knockout mice develop acute myeloid leukemia, suggesting that Nur77 plays a role in regulating differentiation of myeloid progenitor cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human Nur77 recombinant protein (1-266 aa)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IF - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1-0.5 µg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2561762 (BioLegend Cat. No. 653501)
AB_2561763 (BioLegend Cat. No. 653502)

Antigen Details

Structure
64 kD protein containing a DNA-binding domain, two zinc finger domain, and a potential ligand-binding domain
Distribution

Nucleus

Function
Nur77 is a transcription factor that mediates various physiological stimuli, such as growth factors, hormones, inflammatory, and energy metabolic signals. Nur77 plays roles in many important biological processes including cell growth, differentiation, and apoptosis.
Interaction
Interacts with GADD45GIP1 and Bcl-2.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Nuclear Markers, Steroid Receptors/Nuclear Receptors
Antigen References

1. Nakai A, et al. 1990. Mol. Endocrinol. 4:1438.
2. Han YH, et al. 2006. Oncogene 25:2974.
3. Li M, et al. 2010. Mol. Cell. Endocrinol. 319:39.
4. Pearen MA, et al. 2010. Mol. Endocrinol. 24:1891.
5. Moll UM, et al. 2006. Oncogene 25:4725.
6. Fassett MS, et al. 2012. Proc. Natl. Acad. Sci. USA 109:3891.

Gene ID
3164 View all products for this Gene ID
UniProt
View information about Nur77 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 11/30/2018

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