Purified anti-rat CD90 Antibody

Pricing & Availability
Clone
2E11 (See other available formats)
Other Names
Thy-1, Thy1
Isotype
Mouse IgG1, κ
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Product Citations
publications
2E11_PURE_CD90_Antibody_FC_072115
LOU rat thymocytes were stained with purified CD90 (clone 2E11, filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • 2E11_PURE_CD90_Antibody_FC_072115
    LOU rat thymocytes were stained with purified CD90 (clone 2E11, filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
Cat # Size Price Quantity Avail. Save
206101 50 µg $55
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206102 500 µg $175
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Description

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, and stromal cells.

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PC12 cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation and Western blotting.

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 206104) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg)

Application References

(PubMed link indicates BioLegend citation)
  1. Kuroiwa K, et al. 2012. Hybridoma 31:225.
RRID
AB_2565895 (BioLegend Cat. No. 206101)
AB_2565896 (BioLegend Cat. No. 206102)

Antigen Details

Structure
Ig superfamily, GPI-anchored membrane glycoprotein.
Distribution
Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, and glomeruli.
Function
Adhesion, signal transduction, lymphocyte co-stimulation, and proliferation and differentiation of hematopoietic stem cells.
Ligand/Receptor
Interacts with CD45.
Cell Type
Hematopoietic stem and progenitors, Thymocytes, T cells, Neurons, Mast cells, Dendritic cells, B cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/22/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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