- Clone
- 6B9-D6-F8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ribosomal protein S6 kinase alpha-1, 90kD ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, Ribosomal S6 kinase 1, HU-1, P90-RSK1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![6B9-D6-F8_PURE_RSK1_Antibody_011222 6B9-D6-F8_PURE_RSK1_Antibody_011222](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6B9-D6-F8_PURE_RSK1_Antibody_011222-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-RSK1 (clone 6B9-D6-F8) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
Cat # | Size | Price | Quantity Check Availability | Save | ||
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602551 | 25 µg | $118 | ||||
602552 | 100 µg | $293 |
Ribosomal protein S6 kinase α1 (RPS6KA1), most commonly known as RSK1, is a serine/threonine kinase that acts downstream of ERK/MAPK signaling. Upon activation, RSK1 translocates to the nucleus where it controls the activity of several important transcription factors, such as CREB1 and c-FOS. Additionally, RSK1 directly controls translation through phosphorylation of the transcriptional protein RPS6 and EIF4B. Through these roles, RSK1 is involved in the control of cellular growth and proliferation, and genetic variants of RSK1 have been implicated in multiple cancer types.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant RSK1 fragments
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone does not recognize mouse RSK1 in Western Blot
- RRID
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AB_2910476 (BioLegend Cat. No. 602551)
AB_2910476 (BioLegend Cat. No. 602552)
Antigen Details
- Structure
- RSK1 is a 735 amino acid protein with a predicted molecular weight of 83 kD.
- Distribution
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RSK1 is widely expressed in most tissues and cell types with primarily cytosolic localization.
- Function
- Kinase, regulates transcription and translation
- Interaction
- SRF, c-Fos, CREB1, ERK, NFATc4, MITF, ER81
- Biology Area
- Cell Proliferation and Viability, Protein Synthesis, Transcription Factors
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
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- Anjum R, et al. 2008. Nat Rev Cell Biol. 9:747-58.
- Gene ID
- 6195 View all products for this Gene ID
- UniProt
- View information about RSK1 on UniProt.org
Related FAQs
Other Formats
View All RSK1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-RSK1 | 6B9-D6-F8 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-RSK1
Whole cell extracts (15 µg total protein) from the indicated...
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