- Clone
- SP12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Synaptosomal-associated protein 25, SUP, RIC-4, RIC4, SEC9, SNAP
- Previously
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Covance Catalog# MMS-614P
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_SP12_PURE_SNAP25_IHCP_HUCerebellum_113018 A_SP12_PURE_SNAP25_IHCP_HUCerebellum_113018](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/SP12_PURE_SNAP25_IHCP_HUCerebellum_113018.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of purified anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded mouse pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
Western blot of purified anti-SNAP-25 antibody (clone SP12). Lane 1: Molecular weight marker; Lane 2: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
Western blot of purified anti-SNAP-25 antibody (clone SP12). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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805002 | 25 µL | $112 | ||||
805001 | 100 µL | $275 |
SNAP-25, also known as synaptosomal-associated protein 25, is a component of the trans-SNARE complex. It is a Q-SNARE protein anchored to the membrane on the cytosolic face, and does not contain a trans-membrane domain. The SNARE complex is involved in the vesicle fusion process. SNAP-25 is implicated in the inhibition of voltage-gated calcium channels, and has been shown to decrease calcium responsiveness in glutamatergic synapses.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against a crude synaptic preparation from the post mortem human brain.
- Formulation
- Phosphate-buffered solution (no preservatives or carrier proteins).
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
IHC-P - Quality tested
WB - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Honer WG, et al. 1993. Brain Res. 609:9-20.
- Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed
- Product Citations
-
- RRID
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AB_2728531 (BioLegend Cat. No. 805002)
AB_2564674 (BioLegend Cat. No. 805001)
Antigen Details
- Structure
- Expected MW: 28 kD
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Presynaptic proteins
- Gene ID
- 6616 View all products for this Gene ID
- UniProt
- View information about SNAP-25 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All SNAP-25 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-SNAP-25 | SP12 | IHC-P,WB,IP |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-SNAP-25
IHC staining of purified anti-SNAP-25 antibody (clone SP12) ... IHC staining of purified anti-SNAP-25 antibody (clone SP12) ... IHC staining of purified anti-SNAP-25 antibody (clone SP12) ... IHC staining of purified anti-SNAP-25 antibody (clone SP12) ... Western blot of purified anti-SNAP-25 antibody (clone SP12).... Western blot of purified anti-SNAP-25 antibody (clone SP12)....
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