- Clone
- O91D3/SNAP-25 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SNAP-25, Synaptosomal-associated protein 25
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_O91D3slashSNAP25_PU_SNAP25_WB_121118 1_O91D3slashSNAP25_PU_SNAP25_WB_121118](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/O91D3slashSNAP25_PU_SNAP25_WB_121118.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Western blot of purified anti-SNAP-25 antibody (clone O91D3/SNAP-25). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-SNAP-25 antibody (clone O91D3/SNAP-25) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-SNAP-25 antibody (clone O91D3/SNAP-25) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
850301 | 25 µg | $112 | ||||
850302 | 100 µg | $276 |
Synaptosomal-associated protein 25 (SNAP-25) is a component of the trans-SNARE (t-SNARE) complex. SNAP-25 has been reported to account for the specificity of membrane fusion, and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 has been shown to inhibits both ATP-dependent and independent Ca2+-triggered release of glutamate from central nervous system (CNS) synaptosomal membranes. This indicates that SNAP-25 has a role not only in the formation of the synaptic vesicle-target v- and t-SNARE complex, but also in the final neurotransmitter release.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length recombinant human SNAP-25 protein (NP_003072) produced in HEK293T cell.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2715872 (BioLegend Cat. No. 850301)
AB_2715873 (BioLegend Cat. No. 850302)
Antigen Details
- Structure
- SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD.
- Distribution
-
Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei and granule cells of the cerebellum.
Cellular distribution: Cytosol, golgi apparatus and cell membrane. - Function
- SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF).
- Interaction
- SNAP-25 has been found to be part of the SNARE core complex containing SNAP25, VAMP2 and STX1A). SNAP-25 was also found in a complex containing SYT1, SV2B and syntaxin-1. SNAP-25 interacts with KCNB1 (via N-terminus), which reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells. In developing brain and cell cultures, SNAP-25 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture.
- Cell Type
- Mesenchymal Stem Cells, Neurons
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Presynaptic proteins
- Antigen References
-
1. Egbujo CN, et al. 2016. Curr. Psychiatry Rep. 18(8):77.
2. Antonucci F, et al. 2016. Front. Synaptic Neurosci. 8:7.
3. Cupertino RB, et al. 2016. J. Neural. Transm. (Vienna). 123(8):867. - Gene ID
- 6616 View all products for this Gene ID
- UniProt
- View information about SNAP-25 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All SNAP-25 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-SNAP-25 | O91D3/SNAP-25 | WB,IHC-P |
Customers Also Purchased
![](/Files//Images/media_assets/products/product_images/SMI-81_Pure_SNAP25_Antibody_1_081718.png)
![](/Files//Images/media_assets/products/product_images/SP12_PURE_SNAP25_IHCP_HUCerebellum_113018.png)
![](/Files//Images/media_assets/products/product_images/N3569_pure_SVOP_Antibody_1_020918.png)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Follow Us