- Clone
- 7C2C34 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spleen focus forming virus (SFFV) proviral integration oncogene, Sfpi1, Transcription factor PU.1, Dis1, PU.1, Dis-1, Sfpi1, Spi-1, Sfpi-1, Tcfpu1, Tfpu.1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![7C2C34_PURE_SPI1_PU.1_Antibody_1_WB_070918 7C2C34_PURE_SPI1_PU.1_Antibody_1_WB_070918](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/7C2C34_PURE_SPI1_PU1_Antibody_1_WB_070918.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg protein) from THP1 (lane 1) and Raw264.7 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-SPI1 (PU.1) Antibody, clone 7C2C34 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated with HRP for anti-SPI1 (PU.1) Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower) as a loading control. Lane M: Molecular weight ladder. M* Molecular weight ladder with longer exposure. -
Raw 264.7 cells were stained with purified anti-SPI1 (clone 7C2C34) antibody, followed by staining with Alexa Fluor® 594 conjugated goat anti-rat IgG (red) antibody and Alexa Fluor® 488 anti-Tubulin-α antibody (green).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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681301 | 25 µg | $101 | ||||
681302 | 100 µg | $241 |
SPI1 is a transcription factor that belongs to the E26-transformation-specific (ETS) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during the differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as malignancy. The overexpression of SPI1 blocks erythroid differentiation and inhibits cell death. Mice carrying a mutant SPI1 allele shows decreased SPI1 expression and develops acute myeloid leukaemia (AML), which suggests that SPI1 plays a role in oncogenesis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full length SPI1 recombinant protein expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2566468 (BioLegend Cat. No. 681301)
AB_2566518 (BioLegend Cat. No. 681302)
Antigen Details
- Structure
- 272 amino acids with a predicted molecular weight of 31.3 kD. It contains a C-terminal ETS domain that is responsible for DNA binding.
- Distribution
-
Nucleus.
- Function
- SPI1 is a transcription factor that is required for the development of lymphoid and myeloid cells.
- Interaction
- SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmele P, et al. 2010. Cancer Res. 70:6757. - Gene ID
- 20375 View all products for this Gene ID
- UniProt
- View information about SPI1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All SPI1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-SPI1 (PU.1) | 7C2C34 | WB,ICC |
Alexa Fluor® 647 anti-SPI1 (PU.1) | 7C2C34 | ICFC |
Alexa Fluor® 488 anti-SPI1 (PU.1) | 7C2C34 | ICFC |
PE anti-SPI1 (PU.1) | 7C2C34 | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-SPI1 (PU.1)
Total cell lysates (15 µg protein) from THP1 (lane 1) and Ra... Raw 264.7 cells were stained with purified anti-SPI1 (clone ... -
Alexa Fluor® 647 anti-SPI1 (PU.1)
C57BL/6 splenocytes were surface stained with CD19 PE and th... -
Alexa Fluor® 488 anti-SPI1 (PU.1)
C57BL/6 splenocytes were surface stained with CD19 APC and t... -
PE anti-SPI1 (PU.1)
C57BL/6 splenocytes were surface stained with CD19 APC and t...
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