- Clone
- QA18A48 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TAU, MSTD, PPND, DDPAC, MAPT, MTBT1, MTBT2, tau-40, FTDP-17, PPP1R103
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_QA18A48_PURE_Tau269-281_Recom_Antibody_1_022522 1_QA18A48_PURE_Tau269-281_Recom_Antibody_1_022522](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA18A48_PURE_Tau269-281_Recom_Antibody_1_022522.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total tissue lysates (15 µg total protein) from human brain (positive control, Lane 1) and mouse brain (positive control, Lane 2) were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL (1:500) anti-Tau, 269-281 recombinant (clone QA18A48) overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight ladder -
IHC staining of anti-Tau, 269-281 recombinant (clone QA18A48) on formalin-fixed paraffin-embedded human brain (A, positive control) and liver (B, negative control) tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissues were incubated with 5 µg/mL of recombinant anti-Tau, 269-281 overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 μm -
Direct ELISA of anti-Tau, 269-281 recombinant (clone QA18A48) binding to plate-immobilized recombinant human 0N3R (blue) and 0N4R (red) Tau proteins. ELISA was performed by coating wells with 1 µg/mL of Tau proteins. The wells were then incubated with the primary antibody at room temperature for 2 hours, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (3, 3', 5, 5' tetramethylbenzidine) (Cat. No. 421501) Substrate solution was used as the detection system. -
Direct ELISA of anti-Tau, 269-281 recombinant (clone QA18A48) binding to plate-immobilized recombinant human 1N3R (blue) and 1N4R (red) Tau proteins. ELISA was performed by coating wells with 1 µg/mL of Tau proteins. The wells were then incubated with the primary antibody at room temperature for 2 hours, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (3, 3', 5, 5' tetramethylbenzidine) (Cat. No. 421501) Substrate solution was used as the detection system. -
Direct ELISA of anti-Tau, 269-281 recombinant (clone QA18A48) binding to plate-immobilized recombinant human 2N3R (blue) and 2N4R (red) Tau proteins. ELISA was performed by coating wells with 1 µg/mL of Tau proteins. The wells were then incubated with the primary antibody at room temperature for 2 hours, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (3, 3', 5, 5' tetramethylbenzidine) (Cat. No. 421501) Substrate solution was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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948801 | 25 µg | $129 | ||||
948802 | 100 µg | $323 |
Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Recombinant
- Host Species
- Rat
- Immunogen
- Partial recombinant human Tau protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, Direct ELISA - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1 - 25 µg/mL is suggested. For Direct ELISA, the suggested use of this reagent is 0.2 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This antibody recognizes endogenous human and mouse Tau proteins in brain lysates by WB and reacts with 0N3R, 0N4R, 1N3R, 1N4R, 2N3R, and 2N4R recombinant Tau proteins by direct ELISA.
- RRID
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AB_2910513 (BioLegend Cat. No. 948801)
AB_2910513 (BioLegend Cat. No. 948802)
Antigen Details
- Structure
- Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
- Distribution
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Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol. - Function
- Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Interaction
- Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Tau
- Antigen References
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- Yipen W, et al. 2016. Nat Rev Neurosci 17:5-21.
- Dong Y, et al. 2012. PLoS One 7:e39386.
- Augustinack JC, et al. 2002. Acta Neuropathol. 103:26-35.
- Seubert P, et al. 1995. J Biol Chem 270:1817-22.
- Gene ID
- 4137 View all products for this Gene ID
- UniProt
- View information about Tau 269-281 on UniProt.org
Other Formats
View All Tau 269-281 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Tau, 269-281 Recombinant Antibody | QA18A48 | WB,IHC-P,Direct ELISA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Tau, 269-281 Recombinant Antibody
Total tissue lysates (15 µg total protein) from human brain ... IHC staining of anti-Tau, 269-281 recombinant (clone QA18A48... Direct ELISA of anti-Tau, 269-281 recombinant (clone QA18A48... Direct ELISA of anti-Tau, 269-281 recombinant (clone QA18A48... Direct ELISA of anti-Tau, 269-281 recombinant (clone QA18A48...
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