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Purified anti-TFAP2A Antibody

Pricing & Availability
Clone
W20235D (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor AP-2-alpha, AP2-alpha, Activator protein 2, AP-2 transcription factor , AP-2, AP-2 alpha, AP2TF, TFAP2, TFAP2alpha, TFAP2 alpha, TFAP
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W20235D_Purified_TFAP2A_WB_040124
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.125 µg/mL of Purified anti-TFAP2A (clone W20235D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W20235D_Purified_TFAP2A_WB_040124
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.125 µg/mL of Purified anti-TFAP2A (clone W20235D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W20235D_Purified_TFAP2A_IHC-P_040124
    IHC staining of Purified anti-TFAP2A (clone W20235D) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with 0.25 µg/mL (panel A) of Purified anti-TFAP2A (clone W20235D), followed by incubation with 2.5 µg/mL of Alexa Fluor° 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panel B). Images were merged of Purified anti-TFAP2A and DAPI (panel C). Images were captured with a 40X objective. Scale bar: 50 µm
  • C. W20235D_PURE_TFAP2A_IHC-P_040124
    IHC staining of Purified anti-TFAP2A (clone W20235D) on formalin-fixed paraffin-embedded human placenta tissue . Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with (panel A) 1.0 µg/mL of Purified anti-TFAP2A (clone W20235D), followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panel B). Images were merged of Purified anti-TFAP2A and DAPI (panel C). Images were captured with a 40X objective. Scale bar: 50 µm
  • D. W20235D_PURE_TFAP2A_ICC_040124
    Cells were fixed with Fixation Buffer (Cat. No. 420801) and permeabilized with 0.5% Triton X-100 for 10 minutes, and were subsequently blocked with 5% FBS for 1 hour at room temperature. Hep G2 cells (negative control, panel A and C) and HeLa cells (positive control, panel B and D) were then stained with 5 µg/mL of Purified anti-TFAP2A (clone W20235D), followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405422) for 1 hour at room temperature. Cells were also stained with Flash Phalloidin™ Green 488 (Cat. No. 424201) (green) and nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The images were merged for both Hep G2 (panel C) and HeLa cells (panel D). The images were captured by a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 25 µm
  • E. W20235D_PURE_TFAP2A_ICFC_040124
    HeLa cells (positive cell line, filled histogram) and Hep G2 cells (low-expressing cell line, open histogram) were fixed with Fixation Buffer (Cat. No. 420801) and permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-TFAP2A (clone W20235D) or Purified rat IgG2a, κ isotype control (open histogram, dashed line) (Cat No. 400502) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416)
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634351 25 µg $180
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634352 100 µg $495
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Description

TFAP2A, or transcription factor AP-2-alpha, binds to a GC-rich DNA consensus sequence 5'-GCCNNNGGC-3'. Its functions are integral to numerous biological processes including embryonic ectodermal development, stem cell differentiation, and the regulation of cell growth and proliferation. TFAP2A is active in the neural crest and regulates a group of cells in the early embryo that give rise to many tissues and organs, including bones and other tissues of the head and neck. In humans, TFAP2A mutation causes Branchio-oculo-facial syndrome (BOFS). In kidney development, TFAP2A functions in distal nephron development dictating the progression of cells towards a fully differentiated state. TFAP2A is also involved in carcinogenesis and is highly expressed in various types of cancer, including bladder cancer, melanoma, head and neck squamous carcinoma, gastric cancer, ovarian cancer, lung adenocarcinoma, and nasopharyngeal carcinoma. In addition to its role in cancer, TFAP2A is a well-known regulator of craniofacial development and evolution. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human TFAP2A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0625 - 0.5 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.25 - 1.0 µg/mL is suggested. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody (clone W20235D) in immunohistochemistry on formalin-fixed paraffin embedded tissue (IHC-P), antigen retrieval with Tris-EDTA pH 9.0 or Citrate Buffer, 10X (Cat. No. 420902) is recommended. 

For use of this antibody (clone W20235D) in immunocytochemistry (ICC), fixation with either Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X or 100% ice-cold methanol is recommended.

For use of this antibody (clone W20235D) in intracellular flow cytometry (ICFC), fixation with Fixation Buffer (Cat. No. 420801) followed by True-Phos™ Perm Buffer (Cat. No. 425401) is recommended.

This antibody (clone W20235D) is not recommended for mouse or rat reactivity.

Antigen Details

Structure
Forms homodimer or heterodimer with TFAP2B or TFAP2C to bind to DNA. Contains a PPxY motif which aids interaction with WWOX.
Distribution

Highly expressed in placenta, breast and skin. Located in cell nucleus.

Function
A transcription activator binds to the GC-rich sequence to regulate gene expression.
Interaction
WWOX, EP300, CITED2, and CITED4
Biology Area
Cell Biology, Cell Proliferation and Viability, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Milunsky JM, et al. 2008. Am J Hum Genet. 82:1171-7.
  2. Wang WD, et al. 2011. Dev Biol. 360:173-85.
  3. Shi D, et al. 2014. Cancer Prev Res. 7:266-77.
Gene ID
7020 View all products for this Gene ID
UniProt
View information about TFAP2A on UniProt.org

Other Formats

View All TFAP2A Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-TFAP2A W20235D WB,ICC,ICFC,IHC-P
Go To Top Version: 1    Revision Date: 04/01/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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