- Clone
- 10D8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- α-tubulin, Tubulin, alpha-ubiquitous chain
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1. 10D8_PURE_Tubulin-a_Antibody_WB_113017 1. 10D8_PURE_Tubulin-a_Antibody_WB_113017](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/10D8_PURE_Tubulin-a_Antibody_WB_113017.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell lysates (15 µg protein) from HeLa (lane 1), Jurkat (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg /mL) and 1:2500 (0.2 µg /mL) purified anti-Tubulin-α antibody (clone 10D8). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse IgM secondary antibody. 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). -
IHC staining using purified anti-alpha tubulin (clone 10D8) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05 M) with Tween-20 (Cat. No. 925501), the tissue was incubated with 10 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 594 goat anti-mouse IgM antibody (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 μM
Cat # | Size | Price | Quantity Check Availability | Save | ||
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627901 | 25 µg | $101 | ||||
627902 | 100 µg | $218 |
Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant full length human α-tubulin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
ICC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2 - 10 µg/mL is suggested. For immunofluorescent staining applications, a concentration range of 1 - 4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
- Product Citations
-
- RRID
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AB_439760 (BioLegend Cat. No. 627901)
AB_439761 (BioLegend Cat. No. 627902)
Antigen Details
- Structure
- Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
- Distribution
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Ubiquitous
- Function
- Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
- Interaction
- RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
- Modification
- Phosphorylation, acetylation
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
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1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738.
2. Miller FD, et al. 1987. J. Cell Biol. 105:3065.
3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96. - Gene ID
- 84790 View all products for this Gene ID
- UniProt
- View information about Tubulin-alpha on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Tubulin-α Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Tubulin-α | 10D8 | WB,ICC,IHC-P |
Alexa Fluor® 488 anti-Tubulin-α | 10D8 | ICC |
Alexa Fluor® 647 anti-Tubulin-α | 10D8 | ICC |
Alexa Fluor® 594 anti-Tubulin-α | 10D8 | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Tubulin-α
Whole cell lysates (15 µg protein) from HeLa (lane 1), Jurka... IHC staining using purified anti-alpha tubulin (clone 10D8) ... -
Alexa Fluor® 488 anti-Tubulin-α
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten... -
Alexa Fluor® 647 anti-Tubulin-α
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 ... -
Alexa Fluor® 594 anti-Tubulin-α
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 ...
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