- Clone
- 14C2A07 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CLEC15A, MAFA-L, MAFA-2F1, MAFA-LIKE, 2F1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Killer cell lectin-like receptor subfamily G member (KLRG1) is a 30 kD, type II membrane glycoprotein with one C-type lectin domain and one immunoreceptor tyrosine-based inhibitory motif (ITIM). KLRG1 is expressed by subsets of natural killer cells (NKs) and effector and memory T cells. It inhibits cell activation and proliferation and is also a marker of T cell senescence. The binding of KLRG1 to E-, N-, or R- cadherins blocks phosphorylation of AKT and increases the expression of cell cycle inhibitors.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human KLRG1-transfected cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
Antigen Details
- Structure
- Type II membrane glycoprotein, one C-type lectin domain, one ITIM, 30 kD.
- Distribution
-
Subsets of natural killer cells, effector and memory T cells.
- Function
- Inhibits cell activation and proliferation
- Interaction
- AKT.
- Ligand/Receptor
- E-, N-, R- cadherins.
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology
- Antigen References
-
1. Shi L, et al. 2014. J. Immunol. 192:649.
- Gene ID
- 10219 View all products for this Gene ID
- UniProt
- View information about KLRG1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All KLRG1 (MAFA) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human KLRG1 (MAFA) | 14C2A07 | FC |
Brilliant Violet 421™ anti-human KLRG1 (MAFA) | 14C2A07 | FC |
APC anti-human KLRG1 (MAFA) | 14C2A07 | FC |
PerCP/Cyanine5.5 anti-human KLRG1 (MAFA) | 14C2A07 | FC |
PE anti-human KLRG1 (MAFA) | 14C2A07 | FC |
PE/Dazzle™ 594 anti-human KLRG1 (MAFA) | 14C2A07 | FC |
PE/Cyanine7 anti-human KLRG1 (MAFA) | 14C2A07 | FC |
Alexa Fluor® 488 anti-human KLRG1 (MAFA) Antibody | 14C2A07 | FC |
Alexa Fluor® 647 anti-human KLRG1 (MAFA) | 14C2A07 | FC |
APC/Fire™ 810 anti-human KLRG1 (MAFA) | 14C2A07 | FC |
Brilliant Violet 785™ anti-human KLRG1 (MAFA) | 14C2A07 | FC |
APC/Fire™ 750 anti-human KLRG1 (MAFA) | 14C2A07 | FC |
Spark Red™ 718 anti-human KLRG1 (MAFA) (Flexi-Fluor™) | 14C2A07 | FC |
Brilliant Violet 605™ anti-human KLRG1 (MAFA) | 14C2A07 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with purifie... -
Brilliant Violet 421™ anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with CD56 PE... -
APC anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with CD56 PE... -
PerCP/Cyanine5.5 anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with CD56 AP... -
PE anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with CD56 AP... -
PE/Dazzle™ 594 anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with CD56 AP... -
PE/Cyanine7 anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with CD56 Pa... -
Alexa Fluor® 488 anti-human KLRG1 (MAFA) Antibody
Human peripheral blood lymphocytes were stained with CD56 AP... -
Alexa Fluor® 647 anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 810 anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 785™ anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 750 anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human KLRG1 (MAFA) (Flexi-Fluor™)
-
Brilliant Violet 605™ anti-human KLRG1 (MAFA)
Human peripheral blood lymphocytes were stained with anti-hu...
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