TMB High Sensitivity Substrate Solution

Pricing & Availability
Other Names
TMB HS Substrate
Ave. Rating
2 reviews
Description

The TMB High Sensitivity (TMB HS) Substrate solution is an one-step working solution for HRP (horse-radish peroxidase) labeled conjugates. TMB HS substrate solution provides high signal and high sensitivity for ELISA. It is recommended for cytokine ELISA reagent sets. The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2N H2SO4 or 1M H3PO4 (resulting in a yellow color), and read at 450 nm.

Product Details
Cat # Size Price Quantity Avail. Save
421501 60 ml $25.00
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Technical data sheet

Product Details

Storage & Handling
The TMB High Sensitivity Substrate Solution should be stored between 2°C and 8°C. Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures.
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)

1. Zeng S, et al. 2008. Int Immunopharmacol. 10:1707. PubMed
2. Eisenach PA, et al. 2010. J. Cell Sci. 123:4182. PubMed
3. Fan YY, et al. 2012. J Lipid Res. 53:1287. PubMed
4. Querol L, et al. 2014. Neurology. 82:879. PubMed
5. Kiianitsa K, et al. 2104. Nucleic Acids Res. 42:108. PubMed
6. Nogales A, et al. 2014. J Virol. 88:10525. PubMed

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Related FAQs

What is the difference between your two substrate solutions TMB Substrate Reagent Set (Cat#421101) and TMB High Sensitivity Substrate Solution (Cat# 421501)?

TMB Substrate Reagent Set (Cat# 421101) contains two components, which should be mixed immediately prior to use. The TMB High Sensitivity Substrate Solution (Cat# 421501) contains everything in one solution with special stabilizers and is a high kinetic substrate solution which generally gives higher signal. The type of substrate used for an assay can affect the optical density (OD) achievable and the time required to achieve the desired OD.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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