- Clone
- TL2.1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- Toll like receptor 2, TIL4, CD282
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![TL2_1 TL2_1](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/TL2_1.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood monocytes stained with TL2.1 FITC
Cat # | Size | Price | Quantity Check Availability | Save | ||
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309717 | 100 µg | $270 | ||||
309718 | 1 mg | $715 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human TLR2-transfected CHO cells
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
Block, IHC-P, IHC-F, IP, WB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation. Additional reported applications (for the relevant formats) include: inhibition of PGP activity and blocking of cytokine production1,3,7, immunoprecipitation1, immunohistochemistry of 4% paraformaldehyde-fixed frozen sections2 and immunohistochemistry of HOPE-fixed (HEPES-glutamic acid buffer-mediated organic solvent protection effect) paraffin-embedded sections4 , and Western blotting2. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 309716) (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered).
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Application References
(PubMed link indicates BioLegend citation) -
- Flo T, et al. 2000. J. Immunol. 164:2064.
- Faure E, et al. 2001. J. Immunol. 166:2018.
- Sugawara S, et al. 2001. Infect. Immun. 69:4951.
- Droemann D, et al. 2003. Histochem. Cell Biol. 119:103.
- Chavakis E, et al. 2007. Circ. Res. 100:204.PubMed
- Fiala M, et al. 2007. Proc. Natl. Acad. Sci. USA 10.1073/P. Natl. Acad. Sci. USA.0701267104.
- Goo SY, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200.PubMed
- Weiss DJ,et al. 2008.J. Leukoc. Biol. 83:48. PubMed
- Harris, KM., et al. 2011. J. Leukoc Biol. 90:727. PubMed.
- Elass-Rocahrd E, et al. 2012. J Biol Chem. 287:34432. PubMed.
- RRID
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AB_2810467 (BioLegend Cat. No. 309717)
AB_2810468 (BioLegend Cat. No. 309718)
Antigen Details
- Structure
- IL-1R superfamily, TLR family, type 1 transmembrane protein, 90 kD
- Distribution
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Monocytes, granulocytes, endothelial and epithelial cells
- Function
- Activates innate host defense against bacterial pathogens
- Ligand/Receptor
- Microbial lipoproteins, peptidoglycans, lipoteichoic acid
- Cell Type
- Endothelial cells, Epithelial cells, Granulocytes, Monocytes, Neutrophils, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Toll Like Receptors
- Antigen References
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1. Lien E, et al. 1999. J. Biol. Chem. 274:33419.
2. Lien E, et al. 2001. J. Biol. Chem. 276:1873.
3. Sabroe I, et al. 2002. J. Immunol. 168:4701 - Gene ID
- 7097 View all products for this Gene ID
- UniProt
- View information about CD282 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD282 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-human CD282 (TLR2) | TL2.1 | FC,Block,IHC-P,IHC-F,IP,WB |
FITC anti-human CD282 (TLR2) | TL2.1 | FC |
PE anti-human CD282 (TLR2) | TL2.1 | FC |
Alexa Fluor® 488 anti-human CD282 (TLR2) | TL2.1 | FC |
Alexa Fluor® 647 anti-human CD282 (TLR2) | TL2.1 | FC |
Ultra-LEAF™ Purified anti-human CD282 (TLR2) | TL2.1 | FC,Block,IHC-P,IHC-F,IP,WB |
APC anti-human CD282 (TLR2) | TL2.1 | FC |
PE/Cyanine7 anti-human CD282 (TLR2) | TL2.1 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD282 (TLR2)
Human peripheral blood monocytes stained with TL2.1 FITC -
FITC anti-human CD282 (TLR2)
Human peripheral blood monocytes stained with TL2.1 FITC -
PE anti-human CD282 (TLR2)
Human peripheral blood monocytes stained with TL2.1 PE -
Alexa Fluor® 488 anti-human CD282 (TLR2)
Human peripheral blood monocytes stained with TL2.1 Alexa Fl... -
Alexa Fluor® 647 anti-human CD282 (TLR2)
Human peripheral blood monocytes stained with TL2.1 Alexa Fl... -
Ultra-LEAF™ Purified anti-human CD282 (TLR2)
Human peripheral blood monocytes stained with TL2.1 FITC -
APC anti-human CD282 (TLR2)
Human peripheral blood monocytes were stained with CD282 (TL... -
PE/Cyanine7 anti-human CD282 (TLR2)
Human peripheral blood monocytes were stained with anti-huma...
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