- Clone
- S16013N (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD289, TLR9, Toll-like receptor 9
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S16013N_ULEAF_CD289_TLR9_Antibody_1_062321.png S16013N_ULEAF_CD289_TLR9_Antibody_1_062321.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S16013N_ULEAF_CD289_TLR9_Antibody_1_062321.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human 293XL/TLR9 transfected cells were treated with CpG-B only (white bar) or Ultra-LEAF™ anti-human TLR9 (clone S16013N, purple bars), supernatant was harvested the next day and analyzed using the ELISA MAX™ Deluxe Set Human IL-8 (Cat. No. 431504). -
Human peripheral blood mononuclear cells were stained with CD304 Brilliant Violet™ 421 and CD19 APC, fixed, permeabilized, and then intracellularly stained with CD289 (clone S16013N) Ultra-LEAF™ (left) or rat IgG2b, κ Ultra-LEAF™ isotype control (right) followed by anti-rat IgG. Dot plots exclude monocytes. -
Human peripheral blood mononuclear cells were stained with CD304 Brilliant Violet™ 421 and CD19 APC, fixed, permeabilized, and then intracellularly stained with CD289 (clone S16013N) Ultra-LEAF™ (left) or rat IgG2b, κ Ultra-LEAF™ isotype control (right) followed by anti-rat IgG. Dot plots exclude monocytes. -
Human peripheral blood lymphocytes were stained with CD304 Brilliant Violet™ 421, CD19 APC and CD289 (clone S16013N) Ultra-LEAF™ (left) or rat IgG2b, κ Ultra-LEAF™ isotype control (right), followed by anti-rat IgG. Dot plots exclude monocytes. -
Human peripheral blood lymphocytes were stained with CD304 Brilliant Violet™ 421, CD19 APC and CD289 (clone S16013N) Ultra-LEAF™ (left) or rat IgG2b, κ Ultra-LEAF™ isotype control (right), followed by anti-rat IgG. Dot plots exclude monocytes.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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307703 | 100 µg | $281 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen-associated molecular patterns (PAMPs). TLr9 recognizes unmethylated CpG sequences in DNA (i.e. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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Block - Quality tested
FC, ICFC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by blocking. > 80% blocking was observed with 5 µg antibody per well. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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S16013N clone can be used for blocking CpG-activated IL8 cytokine secretion (tested on 293XL/TLR9 cell line) and for intracellular and surface flow cytometry.
- RRID
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AB_2894671 (BioLegend Cat. No. 307703)
Antigen Details
- Distribution
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TLR9 is expressed by B cells and plasmacytoid dendritic cells (pDC).
- Function
- TLR9 recognizes unmethylated CpG sequences in DNA.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Innate Immune Signaling, Toll Like Receptors
- Antigen References
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- Hornung V, et al. 2002. J Immunol. 168:4531
- Eaton-Bassiri A, et al. 2004. Infect Immun. 72:7202.
- Krieg, A. 2007. Clin Invest. 117:1184.
- Decalf J, et al. 2007. J Exp Med. 2423:2437.
- Gene ID
- 54106 View all products for this Gene ID
- UniProt
- View information about CD289 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD289 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Ultra-LEAF™ Purified anti-human CD289 (TLR9) | S16013N | Block,FC,ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human CD289 (TLR9)
Human 293XL/TLR9 transfected cells were treated with CpG-B o... Human peripheral blood mononuclear cells were stained with C... Human peripheral blood mononuclear cells were stained with C... Human peripheral blood lymphocytes were stained with CD304 B... Human peripheral blood lymphocytes were stained with CD304 B...
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