AEC Substrate Buffer

Pricing & Availability
Regulatory Status
RUO
Other Names
Substrate Buffer
Cat # Size Price Quantity Check Availability
925903 50 mL $30.00
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Description

The AEC solution reacts to give a red staining with minimal background noise.

Technical data sheet

Product Details

Storage & Handling
Store this product between 2 and 8°C. AEC preparations should be stored in a manner which minimizes exposure to light.
Application

IHC

Recommended Usage

Each lot of this product is quality control tested by immunohistochemistry.

AEC Substrate Buffer is for use with AEC chromogen concentrate (Cat. No. 925804). The optimal working dilution should be determined for each specific assay condition.

Application Notes

AEC reaction product is soluble in alcohol. Do not use organic solvents after AEC staining.

Protocol:
1. Dilute AEC concentrate (Cat. No. 925804) 1:50 with Substrate Buffer
2. Apply mixture to tissue section
3. Incubate the slides for 5-10 min. at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount using aqueous mounting media
6. AEC induces a red precipitate with almost no background

Antigen Details

Gene ID
NA
Go To Top Version: 0    Revision Date: 03/04/2016

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