Alexa Fluor® 488 anti-human HLA-A,B,C Antibody

Pricing & Availability
Clone
W6/32 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Class I, MHC class I
Isotype
Mouse IgG2a, κ
W632_Alx488_072607
Human peripheral blood lymphocytes stained with W6/32 Alexa Fluor® 488
  • W632_Alx488_072607
    Human peripheral blood lymphocytes stained with W6/32 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability
311415 25 tests $103.00
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311413 100 tests $242.00
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Description

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Cat, Cow, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.

Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Darrow TL, et al. 1989. J. Immunol. 142:3329.
  2. Stern P, et al. 1987. J. Immunol. 138:1088.
  3. Tran TM, et al. 2001. Immunogenetics 53:440.
  4. Barbatis C, et al. 1981. Gut 22:985.
  5. Ayyoub M, et al. 2004. Cancer Immunity 4:7.
  6. DeFelice M, et al. 1990. Cell. Immunol. 126:420.
  7. Fayen J, et al. 1998. Int. Immunol. 10:1347.
  8. Turco MC, et al. 1988. J. Immunol. 141:2275.
  9. Geppert TD, et al. 1989. J. Immunol. 142:3763.
  10. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  11. Nagano M, et al. 2007. Blood 110:151.
  12. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed
  13. Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed
  14. Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed
  15. Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed
  16. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  17. Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79.
  18. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope)
  19. Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed
  20. Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed
  21. Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope)
Product Citations
  1. Sheridan MA, et al. 2020. Nat Protoc. 15:3441. PubMed
  2. Karvas RM, et al. 2022. Cell Stem Cell. 29:810. PubMed
  3. Stopfer LE, et al. 2020. Nat Commun. 2760:11. PubMed
  4. Sparbier CE, et al. 2023. Nat Cell Biol. 25:258. PubMed
  5. Bretscher D 2012. Mol Biol Cell. 1.445138889. PubMed
  6. Sheridan MA, et al. 2021. Development. 148:. PubMed
  7. Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
  8. Turco MY, et al. 2020. Nature Protocols. 19(5):568-577. PubMed
  9. Nobutani K, et al. 2015. PLoS One. 10:130032. PubMed
  10. Johnson D, et al. 2016. Nat Commun. 7:10582. PubMed
  11. Yeong J, et al. 2019. J Immunother Cancer. 7:34. PubMed
  12. Burr ML, et al. 2019. Cancer Cell. 36:385. PubMed
  13. Goodridge J, et al. 2013. J Immunol. 191:1567. PubMed
  14. Eisemann T, et al. 2019. Neuro Oncol. 1.101388889. PubMed
  15. Simmons SK, et al. 2022. Nat Biotechnol. :. PubMed
  16. Lee J, et al. 2014. Proc Natl Acad Sci U S A. 111:364. PubMed
  17. Luo N, et al. 2018. Nat Commun. 0.547222222. PubMed
  18. Larsen BM, et al. 2021. Cell Reports. 36(4):109429. PubMed
  19. Lowes L, et al. 2014. J Vis Exp. 84: 51248. PubMed
RRID
AB_493134 (BioLegend Cat. No. 311415)
AB_493133 (BioLegend Cat. No. 311413)

Antigen Details

Structure
Ig superfamily
Distribution

All nucleated cells

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
3105 View all products for this Gene ID
UniProt
View information about HLA-A on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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