Alexa Fluor® 700 anti-human Ig light chain λ Antibody

Pricing & Availability
Clone
MHL-38 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin light chain lambda
Isotype
Mouse IgG2a, κ
MHL-38_A700_IgLightChaingamma_Antibody_101119_updated
Human peripheral blood lymphocytes were costained with CD19 FITC and Ig Light Chain λ (clone MHL-38) Alexa Fluor® 700 (left) or mouse IgG2a, κ Alexa Fluor® 700 isotype control (right).
  • MHL-38_A700_IgLightChaingamma_Antibody_101119_updated
    Human peripheral blood lymphocytes were costained with CD19 FITC and Ig Light Chain λ (clone MHL-38) Alexa Fluor® 700 (left) or mouse IgG2a, κ Alexa Fluor® 700 isotype control (right).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
316631 25 tests $136.00
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316632 100 tests $270.00
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Description

The MHL-38 antibody reacts with both soluble and membrane human immunoglobulin light chain lambda (λ). It does not react with human immunoglobulin light chain kappa (κ) or heavy chains. The MHL-38 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone MHL-38 is not compatible with Human TruStain FcX (Fc Receptor Blocking Solution) (Cat. No. 422301 & 422302).

RRID
AB_2819949 (BioLegend Cat. No. 316631)
AB_2819950 (BioLegend Cat. No. 316632)

Antigen Details

Structure
Ig family
Distribution

B cells

Cell Type
B cells
Biology Area
Immunology
Gene ID
3535 View all products for this Gene ID
UniProt
View information about Ig light chain lambda on UniProt.org
Go To Top Version: 1    Revision Date: 10/11/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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