Alexa Fluor® 700 anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
A20_Alx700_020608
SJL mouse splenocytes stained with A20 Alexa Fluor® 700
  • A20_Alx700_020608
    SJL mouse splenocytes stained with A20 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
110723 25 µg $101.00
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110724 100 µg $229.00
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
  2. Withers D, et al. 2009. J Immunol. 183:5079. PubMed
  3. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  4. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  5. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  6. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  7. Lucca L, et al. 2014. J Immunol. 193:3267. PubMed
  8. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  9. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  10. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  11. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  12. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  13. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  14. Baldwin SL, et al. 2021. PLoS One. 16:e0247990. PubMed
  15. Park JH, et al. 2022. STAR Protoc. 3:101607. PubMed
  16. Garidou L, et al. 2012. J Virol. 86:7060. PubMed
  17. Semmrich M, et al. 2011. Mucosal Immunol. 0.3125. PubMed
  18. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  19. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  20. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  21. Huang W, et al. 2014. J Immunol. 193:2267. PubMed
  22. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  23. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  24. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  25. Zenke S, et al. 2020. Immunity. 52(2):313-327. PubMed
  26. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  27. Rengarajan S, et al. 2020. Cell Rep Med. :1. PubMed
  28. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  29. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  30. Ng SS, et al. 2020. Nat Immunol. 21:1205. PubMed
  31. Baasch S, et al. 2021. Cell. . PubMed
  32. Tondini E, et al. 2022. NPJ Vaccines. 7:64. PubMed
  33. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  34. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  35. Zhang J, et al. 2021. Nature. 590:457. PubMed
  36. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  37. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  38. Li C, et al. 2018. Cell. 174:285. PubMed
  39. Yabas M, et al. 2011. PLoS One. 6:e26440. PubMed
  40. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  41. Huang W, et al. 2014. J Leukoc Biol. 96:55. PubMed
  42. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  43. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  44. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  45. Li J, et al. 2021. Cell Rep. 37:110124. PubMed
  46. Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed
  47. Senatus L, et al. 2020. JCI Insight. 5:00. PubMed
  48. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  49. Eftychi C et al. 2019. Immunity. 51(2):367-380 . PubMed
  50. Swarnalekha N, et al. 2021. Sci Immunol. 6:. PubMed
  51. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  52. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  53. Schuran FA, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed
RRID
AB_493732 (BioLegend Cat. No. 110723)
AB_493733 (BioLegend Cat. No. 110724)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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