Alexa Fluor® 700 anti-mouse CD45 Antibody

Pricing & Availability
Clone
I3/2.3 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, λ
I3-2dot3_A700_CD45_Antibody_090618
C57BL/6 mouse splenocytes were stained with CD45 (clone I3/2.3) Alexa Fluor® 700 (filled histogram) or rat IgG2b Alexa Fluor® 700 isotype control (open histogram).
  • I3-2dot3_A700_CD45_Antibody_090618
    C57BL/6 mouse splenocytes were stained with CD45 (clone I3/2.3) Alexa Fluor® 700 (filled histogram) or rat IgG2b Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
147715 25 µg $94.00
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147716 100 µg $223.00
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse lymphoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin embedded sections1 and frozen tissue sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Kliment C, et al. 2009. J. Mol. Cell Cardiol. 47:730. (IHC)
  2. Reynolds JM, et al. 2007. J. Immunol. 179:313. (IHC)
Product Citations
  1. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  2. Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed
  3. Alghamri MS, et al. 2022. ACS Nano. 16:8729. PubMed
  4. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  5. Hu X, et al. 2023. Nat Biotechnol. . PubMed
  6. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  7. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  8. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  9. Ni C, et al. 2022. Nat Commun. 13:5919. PubMed
  10. Zhou Z, et al. 2022. Life (Basel). 12:. PubMed
  11. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  12. Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
  13. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  14. Kung YJ, et al. 2022. J Biomed Sci. 29:57. PubMed
RRID
AB_2750448 (BioLegend Cat. No. 147715)
AB_2750449 (BioLegend Cat. No. 147716)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS and Thomas ML. 1994. Annu. Rev. Immunol. 12:85. 
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 2    Revision Date: 06/14/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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