Biotin anti-β-Amyloid, aggregated Antibody

Pricing & Availability
Clone
A17171C (See other available formats)
Regulatory Status
RUO
Other Names
Purified anti-β-Amyloid, AAA, ABETA, ABPP, AD1, APPI, APP, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide
Isotype
Rat IgG2a, κ
A17171C_Biotin_beta-amyloid_Antibody_1_010620
IHC staining of Biotin anti-β-Amyloid, aggregated antibody (clone A17171C) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using formic acid, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • A17171C_Biotin_beta-amyloid_Antibody_1_010620
    IHC staining of Biotin anti-β-Amyloid, aggregated antibody (clone A17171C) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using formic acid, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
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856505 25 µg $153.00
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856506 100 µg $347.00
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Description

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human Abeta S26C dimer.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810721 (BioLegend Cat. No. 856505)
AB_2810722 (BioLegend Cat. No. 856506)

Antigen Details

Structure
Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Interaction
Tau, prion, α2-macroglobulin, apoE and apoJ, toll-like receptor 2 (TLR2), TLR4, TLR6, and their co-receptors CD14, CD36, and CD47.
Ligand/Receptor
Receptor for advanced glycation end products (RAGE), low-density lipoprotein receptor-related protein 1 (LRP1), glycoprotein 330 (gp330/megalin), P-glycoprotein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Selkoe DJ. 1994. Annu. Rev. Cell Biol 10:373-403.
  2. O'Brien RJ, Wong PC. 2011. Annu. Rev. Neurosci. 34:185-204.
  3. Chen GF, et al. 2017. Acta. Pharmacol. Sin. 38:1205-1235.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid on UniProt.org
Go To Top Version: 3    Revision Date: 01/06/2020

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