Biotin anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
11-26c.2a_Biotin_IgD_Antibody_FC_1_041714.jpg
C57BL/6 splenocytes were stained with CD45R/B220 APC and biotinylated IgD (clone 11-26c.2a) (top) or biotinylated rat IgG2a, κ isotype control (bottom), followed by Sav-PE.
  • 11-26c.2a_Biotin_IgD_Antibody_FC_1_041714.jpg
    C57BL/6 splenocytes were stained with CD45R/B220 APC and biotinylated IgD (clone 11-26c.2a) (top) or biotinylated rat IgG2a, κ isotype control (bottom), followed by Sav-PE.
  • 11-26c.2a_Biotin_IgD_Antibody_FC_2_041714.jpg
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Cat # Size Price Quantity Check Availability
405733 50 µg $77.00
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405734 500 µg $253.00
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Description

Surface IgD is an important B cell differentiation marker.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  2. Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed
  3. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  4. Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed
  5. Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed
RRID
AB_2563343 (BioLegend Cat. No. 405733)
AB_2563344 (BioLegend Cat. No. 405734)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 1    Revision Date: 04/22/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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