Brilliant Violet 421™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 421™
SAV_BV421_071911
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
  • SAV_BV421_071911
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability
405226 100 µL $193.00
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405225 100 µg $462.00
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 421™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 421™ by 405 nm laser light induces a fluorescence maximum emission of 421 nm.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Cat. No. 405225: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Cat. No. 405226: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
405225 is bottled at 0.5 mg/ml.
405226 is bottled at 0.1 mg/ml.
(concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
ICC, IHC-F, IHC-P
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 421™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 421™ conjugation. For immunofluorescent staining, we recommend using Cat. No. 405226 at dilutions ≤ 0.015 µg in 100 µL staining volume per million cells. For applications requiring high concentration of streptavidin reagent such as tetramer labeling, we recommend using Cat. No. 405225. It is recommended that the reagent be titrated for optimal performance for each application.

* Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Streptavidin-Brilliant Violet 421™ is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Brilliant Violet 421™ is 340 kD and Streptavidin alone is 52 kD.

Additional Product Notes

View more applications data using this product in immunofluorescence microscopy and labeling tetramers in flow cytometry in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Kim G, et al. 2013. J. Immunol. 190:1510. PubMed.
  2. Giltiay NV, Et al. 2013. J. Immunol. 191:2155. PubMed
  3. Sugiyama D, et al. 2013. PNAS. 110:17945. PubMed
  4. Scally SW, et al. 2013. J. Exp Med. 210:2569. PubMed
  5. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  6. Higdon LE, et al. 2014. PNAS. 111:5652. PubMed
  7. Friedman RS, et al. 2014. PNAS. 111:9223. PubMed
  8. van de Weijer ML, et al. 2014. Nat Commun. 5:3832. PubMed
  9. Mise-Omata S, et al. 2014. Int Immunol. PubMed
  10. Choo JA, et al. 2014. J Virol. 88:10613. PubMed
  11. Xing YL, et al. 2014. J Neurosci. 34:14128. PubMed
  12. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  13. Poh Cm, et al. 2014. Infect Immun. 82:4854. PubMed
  14. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
  15. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  16. Krishnaswamy JK, et al. 2015. PNAS. 112:3056. PubMed
  17. Rampuria P, et al. 2015. Int Immunol. 27:253. PubMed
Product Citations
  1. Angkasekwinai P, et al. 2017. PLoS One. 10.1371/journal.pone.0184684. PubMed
  2. van Eeden C, et al. 2018. Cell Rep. 25:3123. PubMed
  3. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  4. Steines L, et al. 2021. Front Immunol. 12:657894. PubMed
  5. García-Alegria E, et al. 2021. STAR Protoc. 2:100367. PubMed
  6. Reyes RA, et al. 2021. PLoS One. 16:e0261656. PubMed
  7. Combes AJ, et al. 2022. Cell. 185:184. PubMed
  8. He T, et al. 2022. Liver Int. 42:1287. PubMed
  9. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  10. Wolf EW, et al. 2022. Proc Natl Acad Sci U S A. 119:e2201794119. PubMed
  11. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  12. Lei X, et al. 2022. J Exp Med. 219: . PubMed
  13. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  14. Wang Z, et al. 2022. J Exp Med. 219: . PubMed
  15. Buckner CM, et al. 2022. Cell. 185:4333. PubMed
  16. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  17. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
  18. Touizer E, et al. 2023. iScience. 26:105862. PubMed
  19. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  20. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  21. Guo Q, et al. 2023. Front Public Health. 10:1067342. PubMed
  22. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  23. Zhang S, et al. 2023. J Clin Med. 12: . PubMed
  24. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  25. Takizawa F, et al. 2016. J Immunol. 196: 4522 - 4535. PubMed
  26. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  27. Sun P, et al. 2022. iScience. 25:105202. PubMed
  28. Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed
  29. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  30. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
  31. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
  32. Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed
  33. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  34. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  35. Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed
  36. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
  37. Sigmund EC, et al. 2023. PLoS One. 18:e0285597. PubMed
  38. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  39. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  40. Parra–Sánchez H, et al. 2019. Viruses. 1.153472222. PubMed
  41. Miura M, et al. 2019. Wellcome Open Res. 3:105. PubMed
  42. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  43. Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
  44. Hayashi M, et al. 2017. EBioMedicine. 15:127-136. PubMed
  45. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  46. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  47. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  48. Xiong W, et al. 2021. Int J Mol Sci. 22:. PubMed
  49. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  50. Xing Y, et al. 2014. J Neurosci. 34:14128. PubMed
  51. Sei J, et al. 2016. PLoS One. 11: 0152192. PubMed
  52. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  53. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  54. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  55. Mitra T, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.06.035. PubMed
  56. Poon MML, et al. 2021. Sci Immunol. 6:eabl9105. PubMed
  57. Trivedi S, et al. 2015. Vaccine. 33: 7315-7327. PubMed
  58. Sugiyama D, et al. 2013. Proc Natl Acad Sci U S A. 110:17945. PubMed
  59. Giltiay N, et al. 2013. J Immunol. 191:2155. PubMed
  60. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  61. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  62. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  63. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  64. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  65. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  66. Gaudette BT, et al. 2021. J Clin Invest. 131:. PubMed
  67. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  68. Im S, et al. 2016. Nature. 537:417-421. PubMed
  69. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  70. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  71. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  72. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  73. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  74. Zhang R, et al. 2021. MAbs. 13:1904546. PubMed
  75. Koay H, et al. 2016. Nat Immunol. 10.1038/ni.3565. PubMed
  76. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  77. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  78. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  79. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  80. Krishnaswamy J, et al. 2015. Proc Natl Acad Sci U S A. 112:3056. PubMed
  81. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  82. Rowan A, et al. 2016. PLoS Pathog. 12:e1006030. PubMed
  83. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  84. Tarke A, et al. 2022. Cell. 185:847. PubMed
  85. Bähr A, et al. 2016. PLoS One. 11: 0155676. PubMed
  86. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  87. Liu B, et al. 2015. J Immunol. 194:3583. PubMed
  88. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  89. Cavazzoni CB, et al. 2022. Cell Rep. 38:110399. PubMed
  90. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  91. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  92. Roberts E, et al. 2016. PLoS One. 11:e0168488. PubMed
  93. Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed
  94. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  95. Weijer M, et al. 2014. Nat Commun. 5:3832. PubMed
  96. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  97. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  98. Borroto A, et al. 2022. Front Immunol. 13:799919. PubMed
  99. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  100. Sanjo H, et al. 2015. PLoS One. 10: 0128761. PubMed
  101. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  102. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  103. Blomberg R, et al. 2019. Matrix Biol. 83:60. PubMed
  104. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  105. Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed
  106. Tsuzuki S, et al. 2015. Int Immunol. 10.1093/intimm/dxv058. PubMed
  107. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  108. Scally S, et al. 2013. J Exp Med. 210:2569. PubMed
  109. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  110. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed
  111. Wang Z, et al. 2022. J Exp Med. 219:. PubMed
  112. Guelen L, et al. 2022. J Immunother Cancer. 10:. PubMed
  113. Claudepierre M, et al. 2014. J Virol. 88:5242. PubMed
  114. Friedman R, et al. 2014. Proc Natl Acad Sci U S A. 111:9223. PubMed
  115. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  116. Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed
  117. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  118. Lederer K, et al. 2022. Cell. . PubMed
  119. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
  120. Dan JM, et al. 2021. Science. :371. PubMed
  121. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
  122. An X, et al. 2017. PLoS One.. 10.1371/journal.pone.0181904. PubMed
  123. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  124. Fries CN, et al. 2021. Sci Rep. 11:14494. PubMed
  125. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  126. Lang P 2015. Int Immunol. 27:253. PubMed
  127. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  128. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  129. Zhu L, et al. 2016. J Cell Sci. 129: 4238 - 4251. PubMed
  130. Clement RL, et al. 2019. Nat Immunol. 20:1360. PubMed
  131. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  132. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  133. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  134. Wells DK, et al. 2020. Cell. 183:818. PubMed
  135. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  136. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  137. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  138. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  139. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  140. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  141. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  142. Choo J, et al. 2014. J Virol. 88:10613. PubMed
  143. Binnewies M, et al. 2019. Cell. 177:556. PubMed
  144. Cho A, et al. 2022. J Exp Med. 219:. PubMed
  145. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
  146. Giri T, et al. 2022. Heliyon. 8:e09407. PubMed
  147. Higdon L, et al. 2014. Proc Natl Acad Sci U S A. 111:5652. PubMed
  148. Kim G, et al. 2013. J Immunol. 190:1510. PubMed
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