Brilliant Violet 785™ anti-human HLA-A2 Antibody

Pricing & Availability
Clone
BB7.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC-class I
Isotype
Mouse IgG2b, κ
BB72_BV785_HLA-A2_Antibody_1_102617
Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) Brilliant Violet 785™ (filled histograms) or mouse IgG2b Brilliant Violet 785™ isotype control (open histograms).
  • BB72_BV785_HLA-A2_Antibody_1_102617
    Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) Brilliant Violet 785™ (filled histograms) or mouse IgG2b Brilliant Violet 785™ isotype control (open histograms).
  • BB72_BV785_HLA-A2_Antibody_2_102617
Compare all formats See Brilliant Violet 785™ spectral data
Cat # Size Price Quantity Check Availability
343327 25 tests $198.00
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343328 100 tests $418.00
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Description

HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells, playing an important role in cell-mediated immune responses and tumor surveillance.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Papain solubilized HLA-A2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles.

Additional reported applications (for the relevant formats) include: immunoprecipitation3.

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky FM, et al. 1979. Immunol. Rev. 47:3.
  2. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277.
  3. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
Product Citations
  1. Nielsen SS, et al. 2021. EBioMedicine. 68:103410. PubMed
  2. Vibholm LK, et al. 2021. EBioMedicine. 64:103230. PubMed
RRID
AB_2721563 (BioLegend Cat. No. 343327)
AB_2721564 (BioLegend Cat. No. 343328)

Antigen Details

Structure
Ig superfamily
Distribution

A*02 individuals leukocytes

Function
Antigen presentation
Ligand/Receptor
CD8, CD3/TCR
Cell Type
Leukocytes
Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
3105 View all products for this Gene ID
UniProt
View information about HLA-A2 on UniProt.org
Go To Top Version: 0    Revision Date: 10/27/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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