- Regulatory Status
- RUO
- Other Names
- (CEQKLISEEDL), Myc Proto-Oncogene Protein, Proto-Oncogene C-Myc, Transcription Factor P64, Class E Basic Helix-Loop-Helix Protein 39, Avian Myelocytomatosis Viral Oncogene Homolog, V-Myc, Myc-Related Translation/Localization
- Previously
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Covance Catalog# PEP-150P
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 931701 | 1 mg | $213.00 | |||
| 931702 | 5 mg | $433.00 | |||
The c-myc tag is often used as a protein modification in order to simplify the labeling and detection of proteins. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to targeted proteins and allow for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.
Product Details
- Purity
- >90% pure by HPLC
- Formulation
- Lyophilized.
- Storage & Handling
- Store lyophilized or at -20°C in solution
- Application
-
IP, WB
- Recommended Usage
-
Soluble in PBS. The optimal working dilution should be determined for each specific assay condition.
• Elution of epitope tag fusion proteins from immunoaffinity columns: 400 ug/mL
• Competitive inhibition of antibody in western or immunostaining: 10-fold molar excess over optimized (low) antibody - Application Notes
-
This synthetic peptide (H2N-CEQKLISEEDL-COOH) corresponds to the epitope detected by the 9E10 monoclonal antibody.
This peptide can be used in competition experiments, or to elute bound c-myc fusion proteins from the 9E10 affinity matrix.
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Application References
(PubMed link indicates BioLegend citation) -
- Enquist-Newman M, et al. 2001. Mol. Biol. Cell. 12: 2601.
- Ferrando A, et al. 2001. Nucleic Acids Res. 29:3685.
Antigen Details
- Biology Area
- Cell Biology
- Gene ID
- 4609 View all products for this Gene ID
- UniProt
- View information about c-Myc on UniProt.org