MojoSort™ Human CD45 Selection Kit

Pricing & Availability
Regulatory Status
RUO
Mojosort_Human_CD45_Selection_Kit_051223
A single-cell suspension mixture of 30% human peripheral blood mononuclear cells (PBMCs) with 70% human colorectal adenocarcinoma cell line HT-29 was prepared for positive selection of CD45+ cells using the MojoSort™ Human CD45 Selection Kit. Cells were stained with APC anti-human CD326 (clone CO17-1A) (Cat. No. 369809) and a cocktail of PE conjugated antibodies against CD3/CD14/CD15/CD19/CD56 (clones UCHT1/HCD14/W6D3/HIB19/HCD56) (Cat. Nos. 300407/325605/323005/302207/318305). Dead cells were excluded by Helix NP™ Blue (Cat. No. 425305).
  • Mojosort_Human_CD45_Selection_Kit_051223
    A single-cell suspension mixture of 30% human peripheral blood mononuclear cells (PBMCs) with 70% human colorectal adenocarcinoma cell line HT-29 was prepared for positive selection of CD45+ cells using the MojoSort™ Human CD45 Selection Kit. Cells were stained with APC anti-human CD326 (clone CO17-1A) (Cat. No. 369809) and a cocktail of PE conjugated antibodies against CD3/CD14/CD15/CD19/CD56 (clones UCHT1/HCD14/W6D3/HIB19/HCD56) (Cat. Nos. 300407/325605/323005/302207/318305). Dead cells were excluded by Helix NP™ Blue (Cat. No. 425305).
Cat # Size Price Quantity Check Availability
480167 10 tests $198.00
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480168 100 tests $595.00
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Description

Human CD45+ expressing cells are either selected or depleted by incubating the sample with anti-human CD45 Biotin Antibody followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD45+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage

Technical data sheet

Kit Contents

Kit Contents

For Cat. No. 480167:

  • 1 vial containing 100 µL of anti-human CD45 Biotin-Antibody
  • 1 vial containing 100 µL of Streptavidin Nanobeads

For Cat. No. 480168:

  • 1 vial containing 1 mL of anti-human CD45 Biotin-Antibody
  • 1 vial containing 1 mL of Streptavidin Nanobeads​

Product Details

Verified Reactivity
Human
Formulation
Cocktail: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Particle: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µL of anti-human CD45 Biotin-Antibody for 1 x 107 cells in 100 µL of buffer.
10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer.

Application Notes

This kit is designed for the positive selection or depletion of CD45+ leukocytes from human tissues or tumors. 

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

Antibody or cocktail dilution to use in column: 4X
Nanobead dilution to use in column: 4X

Go To Top Version: 1    Revision Date: 05/12/2023

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