- Regulatory Status
- RUO
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A single cell suspension from collagenase enzymatically digested C57BL/6 mouse lung tissues was prepared to isolate CD45+ cells using the MojoSort™ Mouse CD45 Nanobeads. Cells were stained with CD45 (clone 30-F11) Alexa Fluor® 647. Dead cells were excluded by 7-AAD.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 480027 | 20 tests | $198.00 | |||
| 480028 | 200 tests | $694.00 | |||
CD45+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD45 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD45+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat# 480027
- 1 vial containing 200 µl of CD45 Nanobeads
For Cat# 488028
- 2 vials containing 1 ml each of CD45 Nanobeads
Product Details
- Verified Reactivity
- Mouse
- Formulation
- Aqueous solution containing BSA and 0.05% sodium azide.
- Preparation
- Antibody-conjugated particles: protein-coated magnetic nanobeads.
- Storage & Handling
- Mouse CD45 Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl Mouse CD45 Nanobeads in 1x107 cells in 100 µl of buffer.
- Application Notes
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This kit is designed for the positive selection or depletion of CD45+ cells from lymphoid or different organ tissues. For depletion of CD45+ cells a higher volume of beads (up to 15 µl) and two separations may be required. Please contact technical support if needed.
Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 10X - Product Citations
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Antigen Details
- Biology Area
- Cell Biology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD45 on UniProt.org