PE anti-human CD137 (4-1BB) Antibody

Pricing & Availability
Clone
4B4-1 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-7
Other Names
4-1BB, ILA, CD137, TNFRSF9
Isotype
Mouse IgG1, κ
4B4-1
PHA stimulated (3 days) peripheral blood mononuclear cells stained with 4B4-1 PE
  • 4B4-1
    PHA stimulated (3 days) peripheral blood mononuclear cells stained with 4B4-1 PE
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309803 25 tests $118.00
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309804 100 tests $252.00
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Description

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ectodomain of recombinant human 4-1BB fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
  2. Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
  3. Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
  4. Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations
  1. Liu T, et al. 2021. Gut. 70:1965. PubMed
  2. Chow A 2023. Immunity. 56(1):93-106.e6. PubMed
  3. Yamaguchi S, et al. 2021. Eur J Immunol. 51:2306. PubMed
  4. Shitaoka K, et al. 2018. Cancer Immunol Res. 6:378. PubMed
  5. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  6. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
  7. Goncharov MM, et al. 2022. Elife. 11:. PubMed
  8. Shin JJ, et al. 2022. J Clin Immunol. :. PubMed
  9. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  10. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  11. Jung JH, et al. 2021. Nat Commun. 12:4043. PubMed
  12. Pavlicek D, et al. 2017. Immun Ageing. 14:22. PubMed
  13. Gordon-Alonso M,et al. 2017. Nat. Commun. 10.1038/s41467-017-00925-6. PubMed
  14. Elias G, et al. 2022. Elife. 11:. PubMed
  15. Prado-Garcia H, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-1979-x. PubMed
  16. Correnti CE, et al. 2020. Nat Struct Mol Biol. 27:342. PubMed
  17. Gao R, et al. 2018. Oncoimmunology. 7:e1438108. PubMed
  18. Tian L, et al. 2022. Clin Cancer Res. 28:201. PubMed
  19. Senger K, et al. 2022. Curr Protoc. 2:e538. PubMed
  20. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID
AB_314782 (BioLegend Cat. No. 309803)
AB_314783 (BioLegend Cat. No. 309804)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 30 kD
Distribution

Activated T cells

Function
T cell costimulation
Ligand/Receptor
4-1BB ligand
Cell Type
T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.

Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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