- Clone
- 621 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- Activation-inducible TNFR, AITR, Glucocorticoid-inducible TNFR, TNFRSF18
- Isotype
- Mouse IgG1, κ
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Human T lymphoma cell line HUT-78 stained with 621 PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 311603 | 25 tests | $141.00 | |||
| 311604 | 100 tests | $304.00 | |||
GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18). GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and is involved in the development of regulatory T cells and to regulate the activity of Th1 subsets.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 311606).
-
Application References
(PubMed link indicates BioLegend citation) -
- Kwon B, et al. 1999. J. Biol. Chem. 274:6056.
- Zhou Z, et al. 2008. Proc Natl Acad Sci USA . 105:5465 PubMed
- Product Citations
-
- RRID
-
AB_314886 (BioLegend Cat. No. 311603)
AB_314887 (BioLegend Cat. No. 311604)
Antigen Details
- Structure
- TNFR superfamily, 234-amino acid type I transmembrane protein, 25 kD
- Distribution
-
Activated lymphocytes
- Function
- Interacts with TRAF's 1-3, but not TRAF 5 or 6, mediates NF-κB activation through the TRAF2/NIK pathway
- Ligand/Receptor
- GITRL
- Cell Type
- Lymphocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.
- Gene ID
- 8784 View all products for this Gene ID
- UniProt
- View information about CD357 on UniProt.org
Other Formats
View All CD357 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD357 (GITR) | 621 | FC |
| Purified anti-human CD357 (GITR) | 621 | FC |
| PE/Cyanine5 anti-human CD357 (GITR) | 621 | FC |
| APC anti-human CD357 (GITR) | 621 | FC |
| Ultra-LEAF™ Purified anti-human CD357 (GITR) | 621 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD357 (GITR)
Human T lymphoma cell line HUT-78 stained with 621 PE -
Purified anti-human CD357 (GITR)
Human T lymphoma cell line HUT-78 stained with purified 621,... -
PE/Cyanine5 anti-human CD357 (GITR)
Human T lymphoma cell line HUT-78 stained with 621 PE/Cyanin... -
APC anti-human CD357 (GITR)
Human T lymphoma cell line HUT-78 stained with 621 APC -
Ultra-LEAF™ Purified anti-human CD357 (GITR)
Human T lymphoma cell line HUT-78 stained with Ultra-LEAF™ p...