PE anti-human HLA-A,B,C Antibody

Pricing & Availability
Clone
W6/32 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Class I, MHC class I
Isotype
Mouse IgG2a, κ
W632_PE_072607
Human peripheral blood lymphocytes stained with W6/32 PE
  • W632_PE_072607
    Human peripheral blood lymphocytes stained with W6/32 PE
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311405 25 tests $98.00
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311406 100 tests $216.00
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Description

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Cat, Cow, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.

Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Darrow TL, et al. 1989. J. Immunol. 142:3329.
  2. Stern P, et al. 1987. J. Immunol. 138:1088.
  3. Tran TM, et al. 2001. Immunogenetics 53:440.
  4. Barbatis C, et al. 1981. Gut 22:985.
  5. Ayyoub M, et al. 2004. Cancer Immunity 4:7.
  6. DeFelice M, et al. 1990. Cell. Immunol. 126:420.
  7. Fayen J, et al. 1998. Int. Immunol. 10:1347.
  8. Turco MC, et al. 1988. J. Immunol. 141:2275.
  9. Geppert TD, et al. 1989. J. Immunol. 142:3763.
  10. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  11. Nagano M, et al. 2007. Blood 110:151.
  12. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed
  13. Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed
  14. Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed
  15. Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed
  16. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  17. Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79.
  18. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope)
  19. Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed
  20. Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed
  21. Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope)
Product Citations
  1. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
  2. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  3. Vidard L, et al. 2023. Immun Inflamm Dis. 11:e749. PubMed
  4. Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed
  5. Hiatt JB, et al. 2022. Clin Cancer Res. 28:4551. PubMed
  6. Zhang X, et al. 2022. Cell Rep. 40:111363. PubMed
  7. Chimienti R, et al. 2022. Cell Rep. 40:111423. PubMed
  8. Fox DB, et al. 2023. NPJ Precis Oncol. 7:25. PubMed
  9. Feola S, et al. 2021. ACS Nano. 15:15992. PubMed
  10. Oshchepkova A, et al. 2019. Micromachines (Basel). 0.9375. PubMed
  11. Meissner T, et al. 2012. Biochem Biophys Res Commun. 418:786. PubMed
  12. Mayer RL, et al. 2022. Nat Commun. 13:6075. PubMed
  13. Mitchell K, et al. 2018. J Exp Med. 215:1709. PubMed
  14. Izumi K, et al. 2021. Immunohorizons. 5:687. PubMed
  15. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  16. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  17. Blank A, et al. 2021. J Pathol. 253:160. PubMed
  18. Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed
  19. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  20. Zhang J, et al. 2022. Int J Mol Sci. 23:. PubMed
  21. Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed
  22. Skora A, et al. 2015. Proc Natl Acad Sci U S A. 112: 9967-9972. PubMed
  23. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  24. Herbring V, et al. 2016. Sci Rep. 6:36907. PubMed
  25. Blees A, et al. 2015. Sci Rep. 5:17341. PubMed
  26. Nazari-Shafti TZ, et al. 2020. Biomolecules. 10:00. PubMed
  27. Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed
  28. Hu W, et al. 2012. Asian J Androl. 14:695. PubMed
  29. Saluzzo S, et al. 2022. STAR Protoc. 3:101470. PubMed
  30. Arakawa A, et al. 2021. J Immunol. 207:2235. PubMed
  31. Singh MV, et al. 2020. J Leukoc Biol. 109:807. PubMed
  32. Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed
  33. Vantourout P, et al. 2014. Sci Transl Med. 6:231. PubMed
  34. Miller MS, et al. 2019. J Biol Chem. 294:19322. PubMed
  35. Palmer CD, et al. 2022. Nat Med. 28:1619. PubMed
  36. Brohl AS, et al. 2021. Cell Rep. 37:110047. PubMed
  37. Chida K, et al. 2021. Biol Pharm Bull. 44:1551. PubMed
  38. Martufi M, et al. 2019. CRISPR J. 2:31. PubMed
  39. Iraqi M, et al. 2020. Front Cell Infect Microbiol. 10:48. PubMed
  40. Merlo A, et al. 2010. J Immunol. 184:5895. PubMed
  41. Javitt A, et al. 2019. Front Immunol. 10:141. PubMed
  42. Lee PC, et al. 2022. J Clin Invest. 132:. PubMed
  43. Strijker JGM, et al. 2021. J Pers Med. 11:. PubMed
  44. Grossmann N, et al. 2014. Nat Commun. 5:5419. PubMed
  45. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  46. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
  47. Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
  48. Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
RRID
AB_314874 (BioLegend Cat. No. 311405)
AB_314875 (BioLegend Cat. No. 311406)

Antigen Details

Structure
Ig superfamily
Distribution

All nucleated cells

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
3105 View all products for this Gene ID
UniProt
View information about HLA-A on UniProt.org
Go To Top Version: 3    Revision Date: 11/20/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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