PE anti-mouse TNF-α Antibody

Pricing & Availability
Clone
MP6-XT22 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha
Isotype
Rat IgG1, κ
MP6-XT22_PE_TNF-a_Antibody_FC_1_100913
PMA + Ionomycin-stimulated C57BL/6 mouse splenocytes (in the presence of monensin) were stained with CD3 APC, fixed, permeabilized and then stained with TNF-α (clone MP6-XT22) PE (top) or rat IgG1, κ PE isotype control (bottom).
  • MP6-XT22_PE_TNF-a_Antibody_FC_1_100913
    PMA + Ionomycin-stimulated C57BL/6 mouse splenocytes (in the presence of monensin) were stained with CD3 APC, fixed, permeabilized and then stained with TNF-α (clone MP6-XT22) PE (top) or rat IgG1, κ PE isotype control (bottom).
  • MP6-XT22_PE_TNF-a_Antibody_FC_2_101413
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability
506305 25 µg $68.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
506306 100 µg $238.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse TNF-α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Detection: The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 & 510804) as the capture antibody.
ELISA Capture: The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF-α (Cat. No. 575209) as the standard.
Flow Cytometry6,11,12:The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.
Neutralization1,5,10,16,17:The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF-α. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse TNF-α bioactivity in vivo and in vitro(Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections7-9 in vivo detection5, immunofluorescence, and immunocytochemistry.
Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
  2. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20
  3. Mo X, et al. 1995. J. Virol. 69:1288.
  4. Sarawar S, et al. 1994. J. Immunol. 153:1246.
  5. Via C, et al. 2001. J. Immunol. 167:6821. (Neut)
  6. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (FC)
  7. Jacobs M, et al. 2000. Immunology 100:494. (IHC)
  8. Marinova-Mutachieva L, et al. 1997. Clin. Exp. Immunol. 107:507. (IHC)
  9. Williams RO, et al. 2000. J. Immunol. 165:7240. (IHC)
  10. Scanga CA, et al. 1999. Infect. Immun. 67:4531. (Neut)
  11. Akilov OE, et al. 2007. J. Leukoc. Biol. 2007;10.1189/jlb.0706439. (FC)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273. PubMed
  14. Wu S, et al. 2005. Neurosci Lett. 394:158. PubMed
  15. Carlson MJ, et al. 2009. Blood 113:1365. PubMed
  16. Shivakumar P, et al. 2017. JCI Insight. 2:e88747 1. PubMed
  17. Kearney CJ, et al. 2017. Cell Death Differ. 10.1038/cdd.2017.94. PubMed
Product Citations
  1. Belk JA, et al. 2022. Cancer Cell. 40:768. PubMed
  2. Akhtar MN, et al. 2022. MBio. 13:e0330921. PubMed
  3. Pan Y, et al. 2023. Adv Sci (Weinh). 10:e2206792. PubMed
  4. Yu L, et al. 2023. Front Oncol. 13:1174713. PubMed
  5. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  6. Xie J, et al. 2023. Mol Ther Oncolytics. 29:61. PubMed
  7. Asrat S, et al. 2014. PLoS Pathog. 10:1004229. PubMed
  8. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  9. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  10. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  11. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  12. Mendonça-Vieira LR, et al. 2021. Viruses. 13:. PubMed
  13. Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed
  14. Schenkel JM, et al. 2021. Immunity. 54:2338. PubMed
  15. Stone ET, et al. 2022. Cell Rep. 38:110388. PubMed
  16. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  17. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  18. West HC, et al. 2022. Cell Rep. 39:110819. PubMed
  19. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  20. Kang IH, et al. 2022. iScience. 25:104818. PubMed
  21. Balood M, et al. 2022. Nature. 611:405. PubMed
  22. Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed
  23. Liu L, et al. 2022. Cell Biosci. 12:193. PubMed
  24. Huseni MA, et al. 2023. Cell Rep Med. 4:100878. PubMed
  25. Caslin HL, et al. 2023. Front Immunol. 13:984859. PubMed
  26. Cheng H, et al. 2023. Nat Metab. 5:314. PubMed
  27. Zander R, et al. 2022. Immunity. 55:475. PubMed
  28. Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed
  29. Liu W, et al. 2020. Mol Ther Oncolytics. 17:350. PubMed
  30. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  31. Wang Y, et al. 2021. Andrologia. 53:e14239. PubMed
  32. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  33. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  34. Wu T, et al. 2021. Mol Ther Oncolytics. 23:420. PubMed
  35. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  36. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  37. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  38. Liu X, et al. 2020. Nature. . PubMed
  39. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  40. Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
  41. Lu Y, et al. 2019. Int J Mol Med. 43:2398. PubMed
  42. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  43. Yang X, et al. 2021. Front Pharmacol. 12:771046. PubMed
  44. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
  45. Liu Q, et al. 2021. Cell Death Dis. 12:240. PubMed
  46. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  47. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  48. Chen D, et al. 2018. Nat Commun. 9:873. PubMed
  49. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  50. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  51. Suzuki M, et al. 2021. Sci Adv. 7:. PubMed
  52. Cabrera-Mora M, et al. 2015. Infect Immun . 83: 3749-3761. PubMed
  53. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  54. Chen X, et al. 2020. Andrologia. 52:e13823. PubMed
  55. Nagarajan D, et al. 2021. Immunol Cell Biol. 99:972. PubMed
  56. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  57. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  58. Ma J, et al. 2021. Curr Protoc. 1:e144. PubMed
  59. Huang J, et al. 2015. Biochem Biophys Res Commun. 463: 551-556. PubMed
  60. Ni P, et al. 2014. Mol Immunol. 62:199. PubMed
  61. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  62. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  63. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  64. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  65. Chang Q, et al. 2020. Scand J Immunol. 92:e12877. PubMed
  66. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  67. Wagle MV, et al. 2021. Nat Commun. 12:2782. PubMed
  68. Tello-Lafoz M, et al. 2021. Immunity. 54(5):1037-1054.e7. PubMed
  69. Gudgeon N, et al. 2022. Cell Rep. 40:111193. PubMed
  70. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  71. Stutz MD, et al. 2021. Immunity. 54(8):1758-1771.e7. PubMed
  72. Xu S, et al. 2021. Immunity. . PubMed
  73. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  74. Knocke S, et al. 2016. Cell Rep. 17:2234-2246. PubMed
  75. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  76. Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed
  77. Hanna CC, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  78. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  79. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  80. Deczkowska A, et al. 2017. Nat Commun. 8:717. PubMed
  81. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  82. Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed
  83. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  84. Stutz MD et al. 2018. Frontiers in immunology. 1.193055556 . PubMed
  85. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  86. Akilov O, et al. 2007. J Leukoc Biol. 81:1188. PubMed
  87. Robles-Oteiza C, et al. 2021. Dis Model Mech. 14:. PubMed
  88. Sjaastad FV, et al. 2020. Front Immunol. 11:1786. PubMed
  89. Fang Y, et al. 2021. J Clin Invest. 131:00:00. PubMed
  90. Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed
  91. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  92. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  93. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  94. Bennion BG, et al. 2019. J Virol. 93. PubMed
  95. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  96. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  97. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  98. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  99. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  100. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  101. Chen L, et al. 2021. Theranostics. 11:6668. PubMed
  102. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  103. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  104. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  105. Liu J, et al. 2016. Cancer Res . 76: 5288 - 5301. PubMed
  106. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  107. Dahlin J, et al. 2012. J Immunol. 189:3869. PubMed
  108. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  109. Iyer RF, et al. 2022. PLoS Pathog. 18:e1010783. PubMed
  110. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  111. Li M, et al. 2020. J Immunother Cancer. 8:00. PubMed
  112. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  113. Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed
  114. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  115. C Khouili S, et al. 2020. Cell Rep. 33:108468. PubMed
  116. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  117. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
  118. Brenner E, et al. 2020. Nat Commun. 11:1335. PubMed
  119. Li H, et al. 2021. Signal Transduct Target Ther. 6:389. PubMed
RRID
AB_315426 (BioLegend Cat. No. 506305)
AB_315427 (BioLegend Cat. No. 506306)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 17.5-150 kD (Mammalian)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.

Regulation
Processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, and cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, and PAF antagonists
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org
Go To Top Version: 2    Revision Date: 10/14/2013

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account