- Regulatory Status
- RUO
- Other Names
- Streptavidin- PE/Fire™, SAv- PE/Fire™ 640, streptavidin PE/Fire™ 640
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Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV- PE/Fire™ 640 (filled histogram), or biotinylated mouse IgG1, κ isotype control, followed with SAV- PE/Fire™ 640 (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405375 | 25 µg | $195.00 | |||
| 405376 | 100 µg | $425.00 | |||
Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- Streptavidin is conjugated with PE/Fire™ 640 under optimal conditions.
- Concentration
- 0.2 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
- Storage & Handling
- The Streptavidin PE/Fire™ 640 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE/Fire™ 640 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm. - Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Internal testing shows that PE Fire™/640 Streptavidin can interact non-specifically with human peripheral blood cells. To minimize non-specific binding of this tandem conjugate, use True-Stain Monocyte Blocker™.
Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org