Purified anti-mouse Folate Receptor β (FR-β) Antibody

Pricing & Availability
Clone
10/FR2 (See other available formats)
Regulatory Status
RUO
Other Names
FR-β
Isotype
Rat IgG2a, κ
10slashFR2_PURE_Folate_Receptor_beta_Antibody_062217
C57BL/6 mice were IP injected with thioglycolate medium and peritoneal macrophages were collected after 4 days. The cells were then stained with FR-β (10/FR2) (filled histogram) or Rat IgG2a, κ isotype control (open histogram), followed by goat-anti-rat IgG PE.
  • 10slashFR2_PURE_Folate_Receptor_beta_Antibody_062217
    C57BL/6 mice were IP injected with thioglycolate medium and peritoneal macrophages were collected after 4 days. The cells were then stained with FR-β (10/FR2) (filled histogram) or Rat IgG2a, κ isotype control (open histogram), followed by goat-anti-rat IgG PE.
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Cat # Size Price Quantity Check Availability
153302 500 µg $382.00
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Description

Folate receptor beta (FR-β) is a member of a family of folate binding receptors that have diverse structural identities but mediate transport of folates into cells. FR-β is a GPI-anchored protein with high affinity for folic acid. It is postulated that this receptor function as folate scavengers when folate supply is low or rapid cell growth requires elevated uptake of folate for methylation reactions including DNA biosynthesis. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Folate receptor β transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Nagai T, et al. 2009. Cancer Immunol Immunother. 1577-86.
Product Citations
  1. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
RRID
AB_2687271 (BioLegend Cat. No. 153302)

Antigen Details

Structure
30 kD, seven disulfide bonds
Distribution

Placenta, monocytes, tissue resident macrophages

Function
Transport of folate into cells
Ligand/Receptor
Folic Acid
Cell Type
Macrophages, Monocytes
Antigen References

1. Elwood PC, 1989, J Biol Chem, 264:14893.
2. Shen F, et al, 1994, Biochemistry, 8;33:1209.
3. Yi YS, 2016, Immune Netw,16:337.

Gene ID
14276 View all products for this Gene ID
UniProt
View information about Folate Receptor beta on UniProt.org
Go To Top Version: 0    Revision Date: 06/23/2017

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