- Regulatory Status
- RUO
- Other Names
- Streptavidin-PURE, SAV-PU, SAV
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405150 | 1 mg | $71.00 | |||
| 405151 | 10 mg | $411.00 | |||
Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.
Product Details
- Formulation
- Each vial contains Streptavidin lyophilized in sodium chloride buffer.
- Preparation
- Reconstitute the content of the vial in distilled water to the desired concentration.
- Storage & Handling
- For long-term storage, store the lyophilized streptavidin at -20°C or -70°C. After reconstitution, store the solution between 2°C and 8°C for up to 2 weeks, or store in aliquots at -20°C for up to 6 months.
- Application
-
FC - Quality tested
ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
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Antigen Details
- Structure
- 52.8 kD tetrameric protein
- Distribution
-
Streptavidin binds to biotin with high affinity
- Ligand/Receptor
- Biotin
- Antigen References
-
1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85. - Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org