- Regulatory Status
- RUO
- Other Names
- Nerve growth factor, NGF, beta-NGF
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 711906 | 100 µg | $253.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
β-NGF is a pluripotent mediator and it exists in many tissues. Secreted β-NGF can bind to tryosine kinase A receptor with higher affinity and to p75 (NTR) with low affinity. β-NGF is well known as an endogenous neurotrophic factor involved in the embryonal development, survival, and regeneration of mammalian sympathetic and sensory neurons. In adults, β-NGF is also involved in the survival and regeneration of neurons in the central nervous system. Involvement of β-NGF in the immune system has also been reported. In vitro cultured B lymphocyte can constitutively secret β-NGF, which can act as an autocrine growth and differentiation factor for B cells. β-NGF can also downregulate the production of IFN-γ by T cells. Recombinant β-NGF has been used in many clinical trials to treat different disease including Alzheimer's disease, diabetic polyneuropathy, and HIV-associated peripheral neuropathy.
Product Details
- Source
- Human β-NGF, amino acids Ser122-Arg239 (Accession# NP_002497), was expressed in E. coli.
- Molecular Mass
- The 118 amino acid recombinant protein has a predicted molecular mass of approximately 13.5 kD. This protein is a disulfide-linked homodimer and the predicted molecular mass is 27 kD. The predicted N-terminal amino acid is Ser.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Concentration
- This product is bottled at the concentration indicated on the vial.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- Assay 1: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg.
- Application
-
Bioassay
Antigen Details
- Structure
- Dimer
- Distribution
-
Hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells, eosinophils
- Function
- Upregulated by glutamate, vitamin D3, IL-6, FGF-basic, astrocyte-specific IL-1, TNF-α, PDGF, TGF-β; downregulated by GABAergic neuronal activity, glucocorticoids, Schwann cell-specific TGF-β
- Interaction
- Neurons, B cells, mast cells, eosinophils
- Ligand/Receptor
- Low-affinity NGF receptor (p75(NTR)); high-affinity NGF receptor (trkA)
- Bioactivity
- Enhances survival, growth, neurotransmitter biosynthesis of sympathetic, sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation, survival B lymphocytes; possible role in allergy, tissue repair
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Levi A and Alemà S. 1991. Annu. Rev. Pharmacol. Toxicol. 31:205.
3. Varon S, et al. 1991. Adv. Exp. Med. Biol. 296:267.
4. Micera A, et al. 2003. Cytokine Growth Factor Rev. 14:369. - Gene ID
- 4803 View all products for this Gene ID
- UniProt
- View information about beta-NGF on UniProt.org