- Regulatory Status
- RUO
- Other Names
- FcgammaRIa, Fc-Gamma RI, Fc-Gamma RIA, IgG Fc Receptor I, Fc Gamma Receptor Ia, High Affinity Immunoglobulin Gamma Fc Receptor I, Fc Fragment Of IgG, High Affinity Ia, Receptor (CD64), Fc Fragment Of IgG Receptor Ia, FCGR1A, FcγR1A
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When Ultra-LEAF™ purified human IgG1 isotype control recombinant antibody (Cat. No. 403501) is immobilized at 0.5 μg/mL, human FcγRI binds in a dose-dependent manner. The EC50 range for this effect is 0.5 - 2.0 ng/mL. HRP anti-His Tag antibody (Cat. No. 652503) was used to detect the binding. -
Stability testing for human FcγRI. Recombinant human FcγRI was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4⁰C (Control). The samples were tested in a binding assay with human IgG1.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 790002 | 10 µg | $94.00 | |||
| 790004 | 25 µg | $194.00 | |||
| 790006 | 100 µg | $698.00 | |||
| 790008 | 500 µg | $1846.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
FcγRI, also known as CD64 and FcR I, is a type I transmembrane protein, belonging to the immunoglobulin superfamily. FcγRI is an Fc receptor that binds to monomeric IgG with high affinity. Human FcγRI binds to human IgG1, IgG3 and IgG4 with higher affinity, but no affinity for IgG2. However, murine FcγRI only binds to mouse IgG2a with high affinity, but it binds to mouse IgG2b and IgG3 with low affinity and no affinity for mouse IgG1. Upon binding to IgG, FcγRI associates with signal-transducing FcR common γ chain, and in turns conducts downstream signaling. Though the Fc γ chain is dispensable to FcγRI ligand-binding capacity, the surface expression of FcγRI and signaling are impaired in Fc γ chain-deficient cells. FcγRI is exclusively expressed on myeloid cells, including monocytes/macrophages, dendritic cells, and inducible on neutrophils and mast cells. The expression can be upregulated by IFN-γ, IL-10 and G-CSF stimulation. Several studies demonstrated that FcγRI plays a role in antigen capture, IgG-induced cellular phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). In FcγRI-deficient mouse macrophages, the IgG2a-induced phagocytosis and antibody-mediated killing were impaired. In addition, acute and chronic inflammatory responses and immune complex-dependent antigen presentation to primed T cells were disturbed in FcγRI-deficient mice.
Product Details
- Source
- Human FcγRI, amino acid Gln16-Pro288 (Accession # P12314), with 6His tag was expressed in CHO cells.
- Molecular Mass
- The 282 amino acid recombinant protein has a predicted molecular mass of approximately 31.6 kD. The DTT-reduced protein and the non-reduced proteins migrate at approximately 50 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When Ultra-LEAF™ purified human IgG1 isotype control recombinant antibody (Cat. No. 403501) is immobilized at 0.5 μg/mL, human FcγRI binds in a dose-dependent manner. The EC50 range for this effect is 0.5 - 2.0 ng/mL. HRP anti-His Tag antibody (Cat. No. 652503) was used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Immunoglobulin family
- Function
- Activation of naïve T cells, induction of cytokine production, cell survival, and expression of anti-apoptotic proteins including Bcl-XL.
- Interaction
- FcγR subunit, src-type kinase Hck and Lyn
- Ligand/Receptor
- IgG
- Bioactivity
- Measured by its ability to bind human IgG1
- Cell Sources
- In human, FcγRI is restricted to monocytes, macrophage, dendritic cells, but inducible on neutrophils and mast cells. In mice, FcγRI is expressed on dendritic cells and on monocytes/macrophages; plasma membrane.
- Cell Type
- Dendritic cells, Macrophages, Mast cells, Neutrophils, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors, Soluble Receptors
- Antigen References
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- van Vugt MJ, et al. 1996. Blood. 87:3593.
- Ravetch JV & Kinet JP. 1991. Annu Rev Immunol. 9:457.
- Perussia B, et al. 1983. J Exp Med. 158:1092.
- Repp R, et al. 1991. Blood. 78: 885.
- Wang AV, et al. 1994. J Exp Med. 180:1165.
- Bruhns P. 2012. Blood. 119:5640.
- Ioan-Facsinay A, et al. 2002. Immunity. 16:391.
- Barnes N, et al. 2002. Immunity. 16:379.
- Gene ID
- 2209 View all products for this Gene ID
- UniProt
- View information about FcgammaRI on UniProt.org