Recombinant Human Slit2-N (carrier-free)

Slit glycoproteins and their Roundabout (ROBO) receptors were originally identified as important axon guidance molecules during the neural development. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signal to axons projecting from the olfactory bulb. In the spinal cord, Slit proteins guide commissural axons by modulating their response to netrin at the floor plate where only commissural axons that crossed the midline respond to Slit2. Slit2 also play a role in branching and arborization of CNS sensory axons, in neuronal cell migration, and in regulating leukocyte migration. Slit2 is expressed primarily in the fetal lung, kidney, and adult spinal cord, and to a lesser extent in adult adrenal gland, thyroid, and trachea. Slit2 is initially synthesized as a 1499 amino acid precursor, which is subsequently cleaved into N-terminal and C-terminal fragments, designated as Slit2-N and Slit2-C respectively. The neurodevelopment related activities are contained only in the N-terminal fragment. Slit2 binds to multiple receptors including ROBO-1, -2, -3, Laminin-1, DAN, Gremlin, and Glypican-1. Slit2 has been shown to promote angiogenesis via ROBO-1 and ROBO-2 in a mouse model of ocular neovascular disease.