- Regulatory Status
- RUO
- Other Names
- Megakaryocyte Colony Stimulating Factor (MKCSF), Myeloproliferative leukemia virus oncogene ligand (Mpl-ligand), Megakaryocyte growth and development factor (MGDF), THPO
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 712602 | 10 µg | $253.00 | |||
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TPO is a key regulator of megakaryocytopoiesis and thrombopoiesis. It signals through the c-mpl receptor and stimulates the proliferation and maturation of megakaryocytes; hence, TPO plays an important role in regulating the level of circulating platelets in vivo. It has also been shown that TPO is able to promote the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitors. Besides its hematopoietic effects, TPO is expressed in the brain where it promotes apoptosis of hypoxia sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling.
Product Details
- Source
- Human TPO, amino acids Ser22-Leu195 (Accession# AAA50553.1), was expressed in E. coli.
- Molecular Mass
- The 174 amino acid recombinant protein has a predicted molecular mass of approximately 18.6 kD. The predicted N-terminal amino acid is Ser.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg.
- Application
-
Bioassay
Antigen Details
- Structure
- Monomer
- Distribution
-
TPO is produced by liver and kidney.
- Function
- TPO regulates megakaryocytopoiesis and thrombopoiesis.
- Interaction
- Megakaryocytes
- Ligand/Receptor
- c-MPL/CD110.
- Cell Type
- Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Avanzi G. et al. 1988. Br. J. Haematol. 69:359.
2. Deutsch V.R. and Tomer A. 2006. Br. J. Haematol. 134:453.
3. Kaushansky K. 2005. J. Clin. Invest. 115:3339.
4. Li J. et al. 1999. Br. J. Haematol. 106:345.
5. Kato T. et al. 1997. Proc. Natl. Acad. Sci. USA 94:4669.
6. Ehrenreich H. et al. 2005. Proc. Natl. Acad. Sci. USA 102:862. - Gene ID
- 7066 View all products for this Gene ID
- UniProt
- View information about Thrombopoietin on UniProt.org