- Regulatory Status
- RUO
- Other Names
- Vascular Adhesion Protein-1 (VAP-1), AOC3, SSAO, HPAO, Copper Amine Oxidase, Membrane Primary Amine Oxidase
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 597502 | 10 µg | $218.00 | |||
VAP-1 is a type II cell adhesion membrane protein belonging to the copper/topaquinone oxidase family. Functionally, VAP-1 is a nonclassical inflammation-inducible endothelial molecule involved in leukocyte-subtype-specific rolling under physiological shear. Molecularly, VAP-1 belongs to a special class of cell surface amino oxidases. The enzymatic reaction itself and the biologically active end products can potentially regulate the adhesive status of the vessel wall. Thus, VAP-1 is an ectoenzyme that has inter-related adhesive and enzymatic functions in regulating physiological trafficking and inflammation. Inhibition of VAP-1 can protect against inflammation related damage to certain injured tissues. This protein is primarily expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Additionally, VAP-1 can function as a significant prognostic marker for certain cancers and cardiovascular diseases.
Product Details
- Source
- Human VAP-1, amino acids (Gly27-Asn763) (Accession# NP_001264660.1), was expressed in CHO cells.
- Molecular Mass
- The 737 amino acid recombinant protein has a predicted molecular mass of approximately 84 kD. Recombinant VAP-1 is a mixture of monomers and disulfide linked homodimers. The predicted N-terminal amino acid is Gly.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophilized
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- Human VAP-1 is able to produce hydrogen peroxide during the oxidation of benzylamine. The specific activity is > 16 pMoles/min/µg of VAP-1.
- Application
-
Bioassay
Antigen Details
- Structure
- Cytokine
- Distribution
-
Endothelial cells
- Function
- VAP-1 plays an important role in the migration of lymphocytes to inflamed tissue and is a prognostic marker for certain cancers and cardiovascular diseases.
- Interaction
- Leukocytes
- Ligand/Receptor
- Siglec-9
- Bioactivity
- Human VAP-1 is able to induce production of hydrogen peroxide during the oxidation of benzylamine.
- Biology Area
- Cell Adhesion, Cell Biology, Signal Transduction
- Antigen References
-
1. Jalkanen S. and Salmi M. 1993. Res. Immunol. 144:746.
2. Pietruczuk M. et al. 2006. Przegl. Lek. 63:220.
3. Jalkanen S. and Salmi M. 2008. Arterioscler. Thromb. Vasc. Biol. 28:18. - Gene ID
- 8639 View all products for this Gene ID
- UniProt
- View information about VAP-1 on UniProt.org