- Regulatory Status
- RUO
- Other Names
- Streptavidin- Spark NIR™, SAv- Spark NIR™ 685, streptavidin Spark NIR™ 685
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Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and biotinylated anti-human CD3 (left) or with biotinylated mouse IgG1, κ isotype control (right), followed with SAV-Spark NIR™ 685.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405371 | 25 µg | $106.00 | |||
| 405372 | 100 µg | $253.00 | |||
Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- Streptavidin is conjugated with Spark NIR™ 685 under optimal conditions.
- Concentration
- 0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
- Storage & Handling
- The Streptavidin Spark NIR™ 685 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark NIR™ 685 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm. - Excitation Laser
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Red Laser (633 nm)
Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org