- Clone
- MIH24 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TRANCE (TNF-related activation-induced cytokine receptor), CD254, RANK-L (receptor activator of NF-κB ligand), OPGL (Osteoprotegerin ligand), SOFA, TNFSF-11
- Isotype
- Mouse IgG2b, κ
- Barcode Sequence
- TCCGTGTTAGTTTGT
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 347511 | 10 µg | $369.00 | |||
Human CD254, also known as RANKL, TRANCE, and OPGL, is a type II transmembrane protein in the TNF superfamily that has two forms: a membrane-anchored protein and a secreted protein. Its extracellular domain is related to TRAIL, FasL, and TNF. CD254 is expressed on osteoblasts and activated T cells. It has been implicated in the regulation of the interactions between T cells and dendritic cells. It plays an important role in osteoblast differentiation and bone resorption. CD254 activates JNK and NF-κB and induces the expression of IL-1, IL-6, IL-12, and IL-15 in dendritic cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- RANKL-transfected L cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Additional reported applications (for the relevant formats of this clone) include: ELISA and immunohistochemical staining of paraffin-embedded tissue sections2.
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) -
- Kanamaru F, et al. 2004. Immunol. Lett. 94:239.
- Mueller A, et al. 2014. Arthritis Res. Ther. 16:R55. (IHC) PubMed
- RRID
-
AB_2810552 (BioLegend Cat. No. 347511)
Antigen Details
- Structure
- A TNF superfamily member.
- Distribution
-
Expressed on osteoblasts and activated T cells.
- Function
- Plays an important role in osteoblast differentiation and bone resorption, regulates the interactions between T cells and dendritic cells, and T cells and B cells.
- Ligand/Receptor
- RANK, OPG.
- Cell Type
- T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokines/Chemokines
- Antigen References
-
1. Wiethe C, et al. 2003. J. Immunol. 171:4121
2. Page G, et al. 2005. Arthritis Rheum. 52:2307
3. Ji JD, et al. 2009. J. Immunol. 183:7223 - Gene ID
- 8600 View all products for this Gene ID
- UniProt
- View information about CD254 on UniProt.org
Other Formats
View All CD254 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD254 (TRANCE, RANKL) | MIH24 | FC,ELISA,IHC-P |
| PE anti-human CD254 (TRANCE, RANKL) | MIH24 | FC |
| APC anti-human CD254 (TRANCE, RANKL) | MIH24 | FC |
| TotalSeq™-A0356 anti-human CD254 (TRANCE, RANKL) | MIH24 | PG |
| TotalSeq™-C0356 anti-human CD254 (TRANCE, RANKL) | MIH24 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD254 (TRANCE, RANKL)
Human RANKL transfected P815 cells stained with MIH24 PE -
PE anti-human CD254 (TRANCE, RANKL)
PHA-stimulated human peripheral blood mononuclear cells (3 d...
Human RANKL transfected P815 cells stained with MIH24 PE 
PHA-stimulated human peripheral blood mononuclear cells (3 d... -
APC anti-human CD254 (TRANCE, RANKL)
Human RANKL transfected P815 cell line was stained with CD25... -
TotalSeq™-A0356 anti-human CD254 (TRANCE, RANKL)
-
TotalSeq™-C0356 anti-human CD254 (TRANCE, RANKL)