- Clone
- TR75-89 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TNFRII, TNFR type II, p75, TNFR80, TNFRSF1B
- Isotype
- Armenian Hamster IgG
- Barcode Sequence
- GAAGCTGTATCCGAA
Cat # | Size | Price | Quantity Check Availability | ||
---|---|---|---|---|---|
113409 | 10 µg | $369.00 |
CD120b is a 75 kD type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type II (TNFRII) or p75. It is expressed on a variety of cells at low levels; the expression is upregulated upon activation. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRAF1 and TRAF2, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- E. coli -expressed mouse Type II TNFR
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1. The TR75-89 antibody does not block TNF-α binding to receptor. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113406) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 113404) or biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) -
- Sheehan KC, et al. 1995. J. Exp. Med. 181:607. (IP)
- Schlaepfer DD, et al. 2007. J. Biol. Chem. 282:17450. (FC)
- Bell JH, et al. 2007. J. Leukoc. Biol. 82:173. (FC)
- RRID
-
AB_2894663 (BioLegend Cat. No. 113409)
Antigen Details
- Structure
- TNFR superfamily, 75 kD
- Distribution
-
Variety of cell types at low levels
- Function
- Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
- Ligand/Receptor
- TNF-α, LT-α (TNF-β)
- Cell Type
- Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Aggarwal BB, et al. 1985. Nature 318 665.
2. Chan FKM, et al. 2000. Science 288:2351.
3. Loetscher H, et al. 1990. Cell 61:351.
4. Rothe J, et al. 1993. Nature 364:798. - Gene ID
- 21938 View all products for this Gene ID
- UniProt
- View information about CD120b on UniProt.org
Other Formats
View All CD120b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse CD120b (TNF R Type II/p75) | TR75-89 | FC |
PE anti-mouse CD120b (TNF R Type II/p75) | TR75-89 | FC |
TotalSeq™-A0893 anti-mouse CD120b (TNF R Type II/p75) | TR75-89 | PG |
TotalSeq™-C0893 anti-mouse CD120b (TNF R Type II/p75) | TR75-89 | PG |
Brilliant Violet 421™ anti-mouse CD120b (TNF R Type II/p75) | TR75-89 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.