- Clone
- TR75-54.7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TNFRII, TNFR type II, p75, TNFR80, TNFRSF1B
- Isotype
- Armenian Hamster IgG
-
C57BL/6 splenocytes stained with purified TR75-54.7, then detected with biotinylated anti-Armenian hamster IgG followed by Sav-PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 113305 | 100 µg | $110.00 | |||
| 113306 | 1 mg | $149.00 | |||
| 113307 | 5 mg | $539.00 | |||
| 113308 | 25 mg | $1408.00 | |||
| 113309 | 50 mg | $2354.00 | |||
| 113310 | 100 mg | $3383.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD120b is a 75 kD type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type II (TNFRII) or p75. It is expressed on a variety of cells at low levels; the expression is upregulated upon activation. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRAF1 and TRAF2, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. The TR75-54.7 antibody has been shown to block ligand-induced receptor signaling.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- E. coli -expressed mouse Type II TNFR
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block, IP, ELISA Capture, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204).
ELISA Capture1: The TR75-54.7 antibody is useful as a capture antibody when paired with the biotinylated TR75-32.4 antibody (Cat. No. 113203/113204) as the detecting antibody.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, and receptor blocking1. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 113305-113310). -
Application References
(PubMed link indicates BioLegend citation) -
- Sheehan KC, et al. 1995. J. Exp. Med. 181:607.
- RRID
-
AB_2832383 (BioLegend Cat. No. 113305)
AB_2832384 (BioLegend Cat. No. 113306)
AB_2832385 (BioLegend Cat. No. 113307)
AB_2832386 (BioLegend Cat. No. 113308)
AB_2876426 (BioLegend Cat. No. 113309)
AB_2832387 (BioLegend Cat. No. 113310)
Antigen Details
- Structure
- TNFR superfamily, 75 kD
- Distribution
-
Variety of cell types at low levels
- Function
- Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
- Ligand/Receptor
- TNF-α, LT-α (TNF-β)
- Cell Type
- Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Aggarwal BB, et al. 1985. Nature 318 665.
2. Chan FKM, et al. 2000. Science 288:2351.
3. Loetscher H, et al. 1990. Cell 61:351.
4. Rothe J, et al. 1993. Nature 364:798. - Gene ID
- 21938 View all products for this Gene ID
- UniProt
- View information about CD120b on UniProt.org
Other Formats
View All CD120b Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CD120b (TNF R Type II/p75) | TR75-54.7 | FC,Block,IP,ELISA Capture,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Ultra-LEAF™ Purified anti-mouse CD120b (TNF R Type II/p75)
C57BL/6 splenocytes stained with purified TR75-54.7, then de...