- Clone
- MC-631 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Stage-Specific Embryonic Antigen-3
- Isotype
- Rat IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MC-631_A488_121508.jpg MC-631_A488_121508.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MC-631_A488_121508.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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NCCIT (human teratocarcinoma cell line) stained with MC-631 Alexa Fluor® 488
Cat # | Size | Price | Quantity Check Availability | Save | ||
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330305 | 25 tests | $165 | ||||
330306 | 100 tests | $370 |
Stage-Specific Embryonic Antigen-3 (SSEA-3) is an internal core structure of glob-series glycolipids (R-Galβ1-3GalNAcβ1-3Galα1-4Galβ1-R). SSEA-3 is expressed on human teratocarcinoma cells (embryonal carcinoma, EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-3 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.
Product DetailsProduct Details
- Reactivity
- Human,Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- 4-8 cell stage mouse embryos
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1.
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Application References
(PubMed link indicates BioLegend citation) -
- Kannagi R, et al. 1983. EMBO 2:2355. (IF)
- Kaufmann B, et al. 2005. Virology 332:189. (ELISA)
- Product Citations
- RRID
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AB_1279441 (BioLegend Cat. No. 330305)
AB_1279440 (BioLegend Cat. No. 330306)
Antigen Details
- Distribution
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Embryonic stemcells
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Gene ID
- 93961 View all products for this Gene ID
- UniProt
- View information about SSEA-3 on UniProt.org
Related Pages & Pathways
Pages
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