- Clone
- HK1.4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphocyte antigen 6 complex, locus C
- Isotype
- Rat IgG2c, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HK1dot4_AF488_071510 HK1dot4_AF488_071510](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HK1dot4_AF488_071510.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
C57BL/6 bone marrow cells stained with anti-mouse Ly-6C, HK1.4 Alexa Fluor® 488
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
128021 | 25 µg | $124 | ||||
128022 | 100 µg | $265 |
Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- L3 cloned CTL cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Clone HK1.4 does not block the binding of clone RB6-8C58.
Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4. -
Application References
(PubMed link indicates BioLegend citation) -
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
- Herold KC, et al. 1990. Diabetes 39:815. (Activ)
- Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
- Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
- Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
- Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
- Product Citations
- RRID
-
AB_10640820 (BioLegend Cat. No. 128021)
AB_10639728 (BioLegend Cat. No. 128022)
Antigen Details
- Structure
- 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
- Distribution
-
Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.
- Cell Type
- Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97. - Gene ID
- 17067 View all products for this Gene ID
- UniProt
- View information about Ly-6C on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Customers Also Purchased
![](/Files//Images/media_assets/products/product_images/1A8_APC_091809.jpg)
![](/Files//Images/media_assets/products/product_images/M1slash70_PE_042706.jpg)
![](/Files//Images/media_assets/products/product_images/1A8_PECy7_092409.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Follow Us